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A Laboratory Guide to the Tight Junction

A Laboratory Guide to the Tight Junction
  • Author : Jianghui Hou
  • Publisher :Unknown
  • Release Date :2020-04-24
  • Total pages :420
  • ISBN : 9780128226438
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Summary : A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. Provides consistent and detailed research methods Covers various cell, tissue and animal models Includes step-by-step guidance from beginner to sophisticated levels

Claudins

Claudins
  • Author : Kursad Turksen
  • Publisher :Unknown
  • Release Date :2011-07-02
  • Total pages :447
  • ISBN : 1617791849
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Summary : Since the initial discovery of the claudins, the field of tight junctions and cell polarity has become increasingly energized, mainly due to the fact that the claudins have provided an opportunity to understand the molecular basis of the tight junction function and their tissue specific roles. In Claudins: Methods and Protocols, experts and pioneers in the field provide an extensive collection of techniques involving these tight junction proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Claudins: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.

A Laboratory Guide to the Mammalian Embryo

A Laboratory Guide to the Mammalian Embryo
  • Author : David K. Gardner,Michelle Lane,Andrew J. Watson
  • Publisher :Unknown
  • Release Date :2004-01-08
  • Total pages :394
  • ISBN : 0195142268
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Summary : Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.

American Journal of Physiology

American Journal of Physiology
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1992
  • Total pages :229
  • ISBN : UOM:39015023785200
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Summary : "The William Townsend Porter memorial volume": v. 158.

The Paracellular Channel

The Paracellular Channel
  • Author : Jianghui Hou
  • Publisher :Unknown
  • Release Date :2018-06-08
  • Total pages :248
  • ISBN : 9780128146361
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Summary : The Paracellular Channel: Biology, Physiology and Disease serves as the first volume to offer a cohesive and unifying picture of the critical functions of paracellular channels (tight junctions) in different tissues. This new class of ion channel utilizes a completely different mechanism to create ion passage pathways across the cell junction. This volume outlines common principles that govern the organization and regulation of these diverse cellular structures, describes the methodology of study, and highlights the pathophysiologic consequence of abnormal structure and functions of the paracellular channels in human diseases. Coverage includes biochemical, biophysical, structural, physiologic analyses of the paracellular channel, and new technologies for recording and characterization. Offers integrated coverage of all key aspects of the paracellular channel, an understudied field that may hold key insights into some of the most mysterious aspects of physiology Targets different levels of expertise, spanning from graduate students, interns and clinical fellows, to seasoned researchers that study functions, regulation and dysfunctions of different tissue barriers Provides a cohesive and unifying picture that describes the critical functions of paracellular channels (tight junctions) in different tissues

Laboratory Guide in Pharmacology

Laboratory Guide in Pharmacology
  • Author : Tom S. Miya,Harald Groth Oxholm Holck
  • Publisher :Unknown
  • Release Date :1973
  • Total pages :136
  • ISBN : UOM:39015072152179
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Summary :

Oral Histology Laboratory Guide

Oral Histology Laboratory Guide
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1978
  • Total pages :73
  • ISBN : UOM:39015060286641
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Summary :

Skin Barrier Function

Skin Barrier Function
  • Author : T. Agner
  • Publisher :Unknown
  • Release Date :2016-02-04
  • Total pages :164
  • ISBN : 9783318055863
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Summary : Although a very fragile structure, the skin barrier is probably one of the most important organs of the body. Inward/out it is responsible for body integrity and outward/in for keeping microbes, chemicals, and allergens from penetrating the skin. Since the role of barrier integrity in atopic dermatitis and the relationship to filaggrin mutations was discovered a decade ago, research focus has been on the skin barrier, and numerous new publications have become available. This book is an interdisciplinary update offering a wide range of information on the subject. It covers new basic research on skin markers, including results on filaggrin and on methods for the assessment of the barrier function. Biological variation and aspects of skin barrier function restoration are discussed as well. Further sections are dedicated to clinical implications of skin barrier integrity, factors influencing the penetration of the skin, influence of wet work, and guidance for prevention and saving the barrier. Distinguished researchers have contributed to this book, providing a comprehensive and thorough overview of the skin barrier function. Researchers in the field, dermatologists, occupational physicians, and related industry will find this publication an essential source of information.

The Impact of Food Bioactives on Health

The Impact of Food Bioactives on Health
  • Author : Kitty Verhoeckx,Paul Cotter,Iván López-Expósito,Charlotte Kleiveland,Tor Lea,Alan Mackie,Teresa Requena,Dominika Swiatecka,Harry Wichers
  • Publisher :Unknown
  • Release Date :2015-04-29
  • Total pages :338
  • ISBN : 9783319161044
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Summary : “Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.

WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction

WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction
  • Author : World Health Organisation
  • Publisher :Unknown
  • Release Date :1999-05-13
  • Total pages :128
  • ISBN : 0521645999
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Summary : This definitive and essential source of reference has been thoroughly up-dated and revised to meet the requirements of all laboratories involved in the analysis of human semen. The book sets out the fundamental laboratory techniques that should be employed in the diagnosis of male infertility. The text includes descriptions of how to construct a conventional semen profile and provides standardized protocols for performing several optional diagnostic procedures. Such techniques are essential in the evaluation of infertile couples and in assessing fertility in men whose sperm production is suppressed by potential anti-fertility compounds or by toxic agents: they are also of interest in forensic medicine and in connection with artificial insemination. Previous editions of this volume have established themselves as the gold standard in the area of fertility investigation and treatment: this new edition continues that tradition and will be the benchmark for setting more rigorous standards for future years.

The Laboratory Guide

The Laboratory Guide
  • Author : Sir Arthur Herbert CHURCH
  • Publisher :Unknown
  • Release Date :1894
  • Total pages :292
  • ISBN : WISC:89043723543
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Summary :

A Visual Analogy Guide to Human Anatomy & Physiology

A Visual Analogy Guide to Human Anatomy & Physiology
  • Author : Paul A. Krieger
  • Publisher :Unknown
  • Release Date :2017-02-01
  • Total pages :572
  • ISBN : 9781617316272
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Summary : The Visual Analogy Guides to Human Anatomy & Physiology, 3e is an affordable and effective study aid for students enrolled in an introductory anatomy and physiology sequence of courses. This book uses visual analogies to assist the student in learning the details of human anatomy and physiology. Using these analogies, students can take things they already know from experiences in everyday life and apply them to anatomical structures and physiological concepts with which they are unfamiliar. The study guide offers a variety of learning activities for students such as, labeling diagrams, creating their own drawings, or coloring existing black-and-white illustrations to better understand the material presented.

Tight Junctions

Tight Junctions
  • Author : Marcelino Cereijido,James M. Anderson
  • Publisher :Unknown
  • Release Date :2001-06-26
  • Total pages :792
  • ISBN : 9781420038538
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Summary : As the first detailed overview of the subject, Tight Junctions brought together diverse perspectives from leading investigators to provide a multidisciplinary overview of the field. Since its publication in 1992, there has been an explosion of information about tight junctions, particularly in the area of molecular structure, cell and molecular biology, genetics and physiology. The field has advanced so fast and so far that it influences many areas of biology and now has practical implications for pharmacology of drug delivery and understanding a wide range of diseases altering epithelial permeability.

Current Topics in Membranes

Current Topics in Membranes
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1992-01-08
  • Total pages :453
  • ISBN : 0080585108
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Summary : Current Topics in Membranes

The Adhesion Molecule FactsBook

The Adhesion Molecule FactsBook
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2000-08-24
  • Total pages :336
  • ISBN : 0080525970
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Summary : This updated and expanded Second Edition of The Adhesion Molecule FactsBook has nearly double the number of entries of the First Edition, and provides a compendium of the major cell surface adhesion molecules. The introductory chapters detail the organization of the data in the entries section, provide a background to the main adhesion molecule families, and inform the reader how to access information on adhesion molecules on the Internet. The entries have been designed to allow the reader to quickly establish the main structure and functional features of each molecule and where to find information. alternative nomenclature tissue distribution and regulation of expression ligands gene organization and chromosomal location protein structure and molecular weights amino acid sequence of the most commonly studied organisms PIR, SWISSPROT, and EMBL/GenBank accession numbers biological function key references

Excellence in Otolaryngology

Excellence in Otolaryngology
  • Author : T. Himi,K. Takano
  • Publisher :Unknown
  • Release Date :2016-04-28
  • Total pages :132
  • ISBN : 9783318056518
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Summary : This volume is dedicated to the 70th anniversary of the Department of Otolaryngology of the Sapporo Medical University. It commemorates this landmark event and honors the department’s rich tradition of excellence. Chapters feature results of clinical and basic research conducted at the university. The carefully selected articles – ten clinical reviews, ten original research papers, and one case report – reflect the wide range of specialty fields within the department. Topics covered include otology, allergy and rhinology, oncology, sleep medicine, and tonsils. This book is a comprehensive, inspiring contribution to the celebration of one of the oldest departments within the Sapporo Medical University. It contains valuable information for new and experienced otolaryngologists, neurotologists, allergologists, and head and neck surgeons.

A Practical Guide to Basic Laboratory Andrology

A Practical Guide to Basic Laboratory Andrology
  • Author : Lars Björndahl,David Mortimer,Christopher L. R. Barratt,Jose Antonio Castilla,Roelof Menkveld,Ulrik Kvist,Juan G. Alvarez,Trine B. Haugen
  • Publisher :Unknown
  • Release Date :2010-04-01
  • Total pages :229
  • ISBN : 9781139482493
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Summary : This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes pertinent quality control considerations, as well as the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. Throughout the handbook the interpretation of laboratory results in the clinical context is highlighted, and safe laboratory practice is emphasized. It is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.

The Startup Owner's Manual

The Startup Owner's Manual
  • Author : Steve Blank,Bob Dorf
  • Publisher :Unknown
  • Release Date :2020-03-17
  • Total pages :608
  • ISBN : 9781119690726
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Summary : More than 100,000 entrepreneurs rely on this book for detailed, step-by-step instructions on building successful, scalable, profitable startups. The National Science Foundation pays hundreds of startup teams each year to follow the process outlined in the book, and it's taught at Stanford, Berkeley, Columbia and more than 100 other leading universities worldwide. Why? The Startup Owner's Manual guides you, step-by-step, as you put the Customer Development process to work. This method was created by renowned Silicon Valley startup expert Steve Blank, co-creator with Eric Ries of the "Lean Startup" movement and tested and refined by him for more than a decade. This 608-page how-to guide includes over 100 charts, graphs, and diagrams, plus 77 valuable checklists that guide you as you drive your company toward profitability. It will help you: • Avoid the 9 deadly sins that destroy startups' chances for success • Use the Customer Development method to bring your business idea to life • Incorporate the Business Model Canvas as the organizing principle for startup hypotheses • Identify your customers and determine how to "get, keep and grow" customers profitably • Compute how you'll drive your startup to repeatable, scalable profits. The Startup Owner's Manual was originally published by K&S Ranch Publishing Inc. and is now available from Wiley. The cover, design, and content are the same as the prior release and should not be considered a new or updated product.

Molecular Biology of the Cell

Molecular Biology of the Cell
  • Author : Bruce Alberts
  • Publisher :Unknown
  • Release Date :2004
  • Total pages :229
  • ISBN : 0815332181
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Summary :

Water Quality Assessments

Water Quality Assessments
  • Author : Deborah V Chapman
  • Publisher :Unknown
  • Release Date :1996-08-22
  • Total pages :648
  • ISBN : 9780419215905
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Summary : This guidebook, now thoroughly updated and revised in its second edition, gives comprehensive advice on the designing and setting up of monitoring programmes for the purpose of providing valid data for water quality assessments in all types of freshwater bodies. It is clearly and concisely written in order to provide the essential information for all agencies and individuals responsible for the water quality.

Endothelial Cell Culture

Endothelial Cell Culture
  • Author : Roy Bicknell
  • Publisher :Unknown
  • Release Date :1996-09-28
  • Total pages :136
  • ISBN : 0521559901
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Summary : Contains information and recipe-style techniques for culturing different types of endothelial cell.