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A Laboratory Guide to the Tight Junction

A Laboratory Guide to the Tight Junction
  • Author : Jianghui Hou
  • Publisher :Unknown
  • Release Date :2020-04-24
  • Total pages :420
  • ISBN : 9780128226438
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Summary : A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. Provides consistent and detailed research methods Covers various cell, tissue and animal models Includes step-by-step guidance from beginner to sophisticated levels

A Laboratory Guide to the Mammalian Embryo

A Laboratory Guide to the Mammalian Embryo
  • Author : David K. Gardner,Michelle Lane,Andrew J. Watson
  • Publisher :Unknown
  • Release Date :2004-01-08
  • Total pages :394
  • ISBN : 0195142268
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Summary : Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.

A Practical Guide to Basic Laboratory Andrology

A Practical Guide to Basic Laboratory Andrology
  • Author : Lars Björndahl,David Mortimer,Christopher L. R. Barratt,Jose Antonio Castilla,Roelof Menkveld,Ulrik Kvist,Juan G. Alvarez,Trine B. Haugen
  • Publisher :Unknown
  • Release Date :2010-04-01
  • Total pages :229
  • ISBN : 9781139482493
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Summary : This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes pertinent quality control considerations, as well as the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. Throughout the handbook the interpretation of laboratory results in the clinical context is highlighted, and safe laboratory practice is emphasized. It is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.

Physician’s Guide to the Laboratory Diagnosis of Metabolic Diseases

Physician’s Guide to the Laboratory Diagnosis of Metabolic Diseases
  • Author : N. Blau,M. Duran,M.E. Blaskovics,K.M. Gibson
  • Publisher :Unknown
  • Release Date :2012-12-06
  • Total pages :716
  • ISBN : 9783642558788
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Summary : This second edition of The Physician's Guide provides paediatricians and other physicians with a unique aid to help them select the correct diagnosis from a bewildering array of complex clinical and laboratory data. Delay and mistakes in the diagnosis of inherited metabolic diseases may have devastating consequences. The guide, which includes a CD-ROM, describes 298 disorders which have been grouped into 35 chapters according to the type of condition. Within each group of disorders, chapters provide tables of pertinent clinical findings as well as reference and pathological values for crucial metabolites. Relevant metabolic pathways and diagnostic flow charts are included. There are three indices to make the book as user-friendly as possible.

A Laboratory Guide to Human Physiology

A Laboratory Guide to Human Physiology
  • Author : Stuart Ira Fox
  • Publisher :Unknown
  • Release Date :1984
  • Total pages :323
  • ISBN : 0697047245
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Summary :

The Paracellular Channel

The Paracellular Channel
  • Author : Jianghui Hou
  • Publisher :Unknown
  • Release Date :2018-06-08
  • Total pages :248
  • ISBN : 9780128146361
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Summary : The Paracellular Channel: Biology, Physiology and Disease serves as the first volume to offer a cohesive and unifying picture of the critical functions of paracellular channels (tight junctions) in different tissues. This new class of ion channel utilizes a completely different mechanism to create ion passage pathways across the cell junction. This volume outlines common principles that govern the organization and regulation of these diverse cellular structures, describes the methodology of study, and highlights the pathophysiologic consequence of abnormal structure and functions of the paracellular channels in human diseases. Coverage includes biochemical, biophysical, structural, physiologic analyses of the paracellular channel, and new technologies for recording and characterization. Offers integrated coverage of all key aspects of the paracellular channel, an understudied field that may hold key insights into some of the most mysterious aspects of physiology Targets different levels of expertise, spanning from graduate students, interns and clinical fellows, to seasoned researchers that study functions, regulation and dysfunctions of different tissue barriers Provides a cohesive and unifying picture that describes the critical functions of paracellular channels (tight junctions) in different tissues

Oral Histology Laboratory Guide

Oral Histology Laboratory Guide
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1978
  • Total pages :73
  • ISBN : UOM:39015060286641
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Summary :

Laboratory Guide to Human Anatomy and Physiology

Laboratory Guide to Human Anatomy and Physiology
  • Author : Stuart Ira Fox
  • Publisher :Unknown
  • Release Date :1986
  • Total pages :499
  • ISBN : 0697005852
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Summary :

General Zoology Laboratory Guide

General Zoology Laboratory Guide
  • Author : Charles F. Lytle,John R. Meyer
  • Publisher :Unknown
  • Release Date :2004-02-28
  • Total pages :372
  • ISBN : 007234900X
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Summary : General Zoology Laboratory Manual is ideal for the laboratory that emphasizes the dissection and microscopic study of live and preserved specimens. Recognized for its accuracy and readability, this manual is comprehensive in its representation of the major groups of animal phyla.This new edition is suitable for a wide range of course needs and structures.

The Laboratory Guide

The Laboratory Guide
  • Author : Sir Arthur Herbert CHURCH
  • Publisher :Unknown
  • Release Date :1894
  • Total pages :292
  • ISBN : WISC:89043723543
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Summary :

The laboratory guide for students of agricultural chemistry

The laboratory guide for students of agricultural chemistry
  • Author : sir Arthur Herbert Church
  • Publisher :Unknown
  • Release Date :1882
  • Total pages :229
  • ISBN : OXFORD:600036634
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Summary :

A Practical Guide to the Histology of the Mouse

A Practical Guide to the Histology of the Mouse
  • Author : Cheryl L. Scudamore
  • Publisher :Unknown
  • Release Date :2013-12-24
  • Total pages :248
  • ISBN : 9781118789476
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Summary : A Practical Guide to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year. Complete phenotypic analysis of all of these models can benefit from histologic review of the tissues. This book is aimed at veterinary and medical pathologists who are unfamiliar with mouse tissues and scientists who wish to evaluate their own mouse models. It provides practical guidance on the collection, sampling and analysis of mouse tissue samples in order to maximize the information that can be gained from these tissues. As well as illustrating the normal microscopic anatomy of the mouse, the book also describes and explains the common anatomic variations, artefacts associated with tissue collection and background lesions to help the scientist to distinguish these changes from experimentally- induced lesions. This will be an essential bench-side companion for researchers and practitioners looking for an accessible and well-illustrated guide to mouse pathology. Written by experienced pathologists and specifically tailored to the needs of scientists and histologists Full colour throughout Provides advice on sampling tissues, necropsy and recording data Includes common anatomic variations, background lesions and artefacts which will help non-experts understand whether histologic variations seen are part of the normal background or related to their experimental manipulation

American Journal of Physiology

American Journal of Physiology
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1992
  • Total pages :229
  • ISBN : UOM:39015023785200
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Summary : "The William Townsend Porter memorial volume": v. 158.

Instructors Resource Guide

Instructors Resource Guide
  • Author : Elaine N. Marieb,Barbara Stewart
  • Publisher :Unknown
  • Release Date :2001-11-02
  • Total pages :229
  • ISBN : 0805364730
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Summary :

Skin Barrier Function

Skin Barrier Function
  • Author : T. Agner
  • Publisher :Unknown
  • Release Date :2016-02-04
  • Total pages :164
  • ISBN : 9783318055863
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Summary : Although a very fragile structure, the skin barrier is probably one of the most important organs of the body. Inward/out it is responsible for body integrity and outward/in for keeping microbes, chemicals, and allergens from penetrating the skin. Since the role of barrier integrity in atopic dermatitis and the relationship to filaggrin mutations was discovered a decade ago, research focus has been on the skin barrier, and numerous new publications have become available. This book is an interdisciplinary update offering a wide range of information on the subject. It covers new basic research on skin markers, including results on filaggrin and on methods for the assessment of the barrier function. Biological variation and aspects of skin barrier function restoration are discussed as well. Further sections are dedicated to clinical implications of skin barrier integrity, factors influencing the penetration of the skin, influence of wet work, and guidance for prevention and saving the barrier. Distinguished researchers have contributed to this book, providing a comprehensive and thorough overview of the skin barrier function. Researchers in the field, dermatologists, occupational physicians, and related industry will find this publication an essential source of information.

Human Neuroanatomy

Human Neuroanatomy
  • Author : J. Edward Bruni,Donald G. Montemurro
  • Publisher :Unknown
  • Release Date :2009
  • Total pages :342
  • ISBN : 9780195371420
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Summary : Human Neuroanatomy: A Text, Brain Atlas, and Laboratory Dissection Guide has been substantially changed and updated from a previous edition entitled The Human Brain in Dissection published in 1988 and accordingly has been re-titled. The last 20 years have seen a significant shift in the way anatomy and its sub-disciplines like neuroanatomy are taught in both undergraduate and graduate neuroscience courses; not only has the time allocated for these courses been reduced, but the teaching methodologies have become more focused and specific due to time constraints. As reported by Drake et. al., "Medical education in the anatomical sciences: the winds of change continue to blow" (Anat. Sci. Educ., 2: 253-259, 2009), we have seen an overall drop in the total number of lecture hours and laboratory hours since the last survey done of medical curricula in 2002. Human Neuroanatomy has been reconstructed to appeal to just these changes: courses with a lab/dissection component as well as those without will find this guide the perfect teaching tool to understand human neuroanatomy. With these limitations in mind and to better meet current requirements the authors have expanded the textural content in this new edition and separated it entirely from the dissection instructions which have been retained. The "Laboratory Exercise" as it is now designated stands alone in a highlighted box in each chapter. It outlines what is to be accomplished during a given session using pre-dissected specimens and/or appropriate models or by exposing them in a dissection. Clear step by step procedural instructions are provided and important structures to be seen are highlighted. The dissection sequence laid out in the chapters is a progressive one requiring only a single wet specimen and ideally completed in two hour periods. Students who do not have the opportunity to dissect, however may simply skip these paragraphs. In this 3rd edition of the book many new illustrations have been added to better depict the salient features of the brain at various stages of dissection and to facilitate understanding the subject matter. Labeling of some illustrations has changed and others have been replaced. All are amply referenced to the text and to the laboratory exercises and are intended to assist with or be used in lieu of dissection. New also in this edition is a section of clinically-relevant notes as well as USMLE type multiple-choice questions added in separate sections at the end of each chapter. These quiz type questions provide students with a means of assessing their understanding of the subject matter in each chapter and an indication of how their knowledge might be tested. And finally, an atlas of 62 labelled brain sections in four different planes, at the end of the book, has been retained. CT scans and M.R. images that correspond as closely as possible to the anatomic section are included. Comprehensive and concise Human Neuroanatomy: A Text, Brain Atlas, and Laboratory Dissection Guide is an invaluable guide to assist medical, dental and allied health science students understand nervous system structure, function and disease.

Hormone-regulated Channel-like Tight Junction Permeability in Malpighian Tubules of Aedes Aegypti

Hormone-regulated Channel-like Tight Junction Permeability in Malpighian Tubules of Aedes Aegypti
  • Author : Ming-Jiun Yu
  • Publisher :Unknown
  • Release Date :2004
  • Total pages :232
  • ISBN : CORNELL:31924100224835
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Summary : Tight junctions, the regions of cell contact at the apical surfaces of epithelia, have been considered rather static structures with fixed permeability. However, evidence for their dynamic physiological behavior has been emerging in recent years. Nowhere can rapid changes in tight junction permeability be better observed than in Malpighian tubules of the yellow fever mosquito, Aedes aegypti. This thesis presents the physiological and molecular evidence consistent with the channel-like properties of tight junctions under a hormonal control. The hormone, leucokinin, increases the Cl- conductance of the tight junction thereby increasing the rates of transepithelial NaCl and KCl secretion and, consequently, the secretion of water. The stimulation of secretory transport may mediate in part the diuresis after the blood meal that rids the mosquito of the unwanted salt and water of the meal.

Alternatives to Laboratory Animals

Alternatives to Laboratory Animals
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1990
  • Total pages :229
  • ISBN : UCAL:B4547440
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Summary :

A Learning System in Histology

A Learning System in Histology
  • Author : Deborah W. Vaughan
  • Publisher :Unknown
  • Release Date :2002
  • Total pages :235
  • ISBN : NWU:35557000186484
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Summary : This CD-ROM with accompanying manual will introduce medical, allied health, and undergraduate students to the logic of anatomical thinking and the microscopic structure of the body's tissues. Its correlation of low power with high power views, the quality of its images, and the ease of usingthe CD are particularly attractive features. It is not a full electronic atlas with hyperlinks of pictures to text but rather a review of morphology that supplements the histology lab where students look through a microscope at slides of tissue specimens.

Holt ChemFile Lab Program

Holt ChemFile Lab Program
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2005
  • Total pages :229
  • ISBN : 0030368014
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Summary :

A Laboratory Guide in Pharmacology

A Laboratory Guide in Pharmacology
  • Author : Torald Hermann Sollmann
  • Publisher :Unknown
  • Release Date :1917
  • Total pages :345
  • ISBN : HARVARD:HC3571
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Summary :