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Advanced Methods in Molecular Biology and Biotechnology

Advanced Methods in Molecular Biology and Biotechnology
  • Author : Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
  • Publisher :Unknown
  • Release Date :2020-11-10
  • Total pages :218
  • ISBN : 012824450X
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Summary : Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Molecular Biology and Biotechnology

Molecular Biology and Biotechnology
  • Author : John M. Walker,Ralph Rapley,E. B. Gingold
  • Publisher :Unknown
  • Release Date :2000
  • Total pages :563
  • ISBN : 9780854046065
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Summary : This volume provides a revised and updated introduction to the techniques of molecular biology and its industrial applications. It should be of particular benefit to undergraduates and researchers in other biological areas.

Molecular Biology Techniques

Molecular Biology Techniques
  • Author : Sue Carson,Susan Carson,Heather Miller,D. Scott Witherow
  • Publisher :Unknown
  • Release Date :2012
  • Total pages :200
  • ISBN : 0123855446
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Summary : This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Computational Methods in Molecular Biology

Computational Methods in Molecular Biology
  • Author : S.L. Salzberg,D.B. Searls,S. Kasif
  • Publisher :Unknown
  • Release Date :1998-06-19
  • Total pages :368
  • ISBN : 9780080860930
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Summary : Computational biology is a rapidly expanding field, and the number and variety of computational methods used for DNA and protein sequence analysis is growing every day. These algorithms are extremely valuable to biotechnology companies and to researchers and teachers in universities. This book explains the latest computer technology for analyzing DNA, RNA, and protein sequences. Clear and easy to follow, designed specifically for the non-computer scientist, it will help biologists make better choices on which algorithm to use. New techniques and demonstrations are elucidated, as are state-of-the-art problems, and more advanced material on the latest algorithms. The primary audience for this volume are molecular biologists working either in biotechnology companies or academic research environments, individual researchers and the institutions they work for, and students. Any biologist who relies on computers should want this book. A secondary audience will be computer scientists developing techniques with applications in biology. An excellent reference for leading techniques, it will also help introduce computer scientists to the biology problems. This is an outstanding work which will be ideal for the increasing number of scientists moving into computational biology.

Advances in Molecular Techniques

Advances in Molecular Techniques
  • Author : Rakesh S. Sengar,Amit Kumar,Reshu Chaudhary,Ashu Singh
  • Publisher :Unknown
  • Release Date :2018-05-11
  • Total pages :436
  • ISBN : 1351021206
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Summary : Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists.This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering.

Molecular Biology Techniques

Molecular Biology Techniques
  • Author : Walt Ream,Katharine G. Field
  • Publisher :Unknown
  • Release Date :1998-11-17
  • Total pages :234
  • ISBN : 0080536824
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Summary : This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

Methods in Biotechnology

Methods in Biotechnology
  • Author : Seung-Beom Hong,M. Bazlur Rashid,Lory Z. Santiago-Vázquez
  • Publisher :Unknown
  • Release Date :2016-05-12
  • Total pages :360
  • ISBN : 1119156793
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Summary : As rapid advances in biotechnology occur, there is a need for a pedagogical tool to aid current students and laboratory professionals in biotechnological methods; Methods in Biotechnology is an invaluable resource for those students and professionals. Methods in Biotechnology engages the reader by implementing an active learning approach, provided advanced study questions, as well as pre- and post-lab questions for each lab protocol. These self-directed study sections encourage the reader to not just perform experiments but to engage with the material on a higher level, utilizing critical thinking and troubleshooting skills. This text is broken into three sections based on level – Methods in Biotechnology, Advanced Methods in Biotechnology I, and Advanced Methods in Biotechnology II. Each section contains 14-22 lab exercises, with instructor notes in appendices as well as an answer guide as a part of the book companion site. This text will be an excellent resource for both students and laboratory professionals in the biotechnology field.

Molecular Techniques in Food Biology

Molecular Techniques in Food Biology
  • Author : Aly Farag El Sheikha,Robert E. Levin,Jianping Xu
  • Publisher :Unknown
  • Release Date :2018-04-09
  • Total pages :472
  • ISBN : 111937460X
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Summary : Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity and Traceability explores all aspects of microbe-food interactions, especially as they pertain to food safety. Traditional morphological, physiological, and biochemical techniques for the detection, differentiation, and identification of microorganisms have severe limitations. As an alternative, many of those responsible for monitoring food safety are turning to molecular tools for identifying foodborne microorganisms. This book reviews the latest molecular techniques for detecting, identifying, and tracing microorganisms in food, addressing both good foodborne microbes, such as those used for fermentation and in probiotics, and harmful ones responsible for foodborne illness and food quality control problems. Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity and Traceability brings together contributions by leading international authorities in food biology from academe, industry, and government. Chapters cover food microbiology, food mycology, biochemistry, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and food science and technology. Throughout, special emphasis is placed on novel molecular techniques relevant to food biology research and for monitoring and assessing food safety and quality. Brings together contributions from scientists at the leading edge of the revolution in molecular food biology Explores how molecular techniques can satisfy the dire need to deepen our understanding of how microbial communities develop in foods of all types and in all forms Covers all aspects of food safety and hygiene, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and more Fills a yawning gap in the world literature on food traceability using molecular techniques This book is an important working resource for professionals in the agricultural, food and biomedical sciences, as well as government personnel involved in food regulation and safety. It is also an excellent reference for advanced students in agriculture, food science and food technology, biochemistry, microbiology, and biotechnology, as well as academic researchers in those fields.

Advanced Molecular Genetics

Advanced Molecular Genetics
  • Author : Alfred Pühler,Kenneth N. Timmis
  • Publisher :Unknown
  • Release Date :2012-12-06
  • Total pages :350
  • ISBN : 3642693059
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Summary : The development of powerful new techniques and refmements of tech niques in molecular genetics in recent years, and the surge in interest in biotechnology based on genetic methods, have heralded a new golden age in molecular genetics, and stimulated in diverse disciplines much interest in the technologies themselves and their potential uses in basic and applied biomedical sciences. Although some excellent specialist laboratory manuals (especially the Cold Spring Harbor Laboratory manuals by I. H. Miller; R. W. Davies et al. ; and T. Maniatis et al. ) on certain chapters of molecular genetics exist, no general text that covers a broad spectrum of the sub ject has thus far been published. The purpose of this manual is to pre sent most, though of necessity not all of the important methods of molecular genetics, in a series of simple experiments, many of which can be readily accomplished by the microbiologist, biochemist or biotechnologist that has had only limited exposure to genetics. The remainder of the experiments require either greater familiarity with the subject, or guidance by someone with such experience. The book should, therefore, not only enable individuals to acquire new proce dures for ongoing projects, but also serve as a basis for the teaching of molecular genetic techniques in formal predoctoral and postdoctoral laboratory courses.

Molecular Biology Techniques

Molecular Biology Techniques
  • Author : Sue Carson,Heather Miller,Melissa C. Srougi,D. Scott Witherow
  • Publisher :Unknown
  • Release Date :2019-03-05
  • Total pages :294
  • ISBN : 0128157755
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Summary : Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions

Methods in Plant Molecular Biology and Biotechnology

Methods in Plant Molecular Biology and Biotechnology
  • Author : Bernard R. Glick
  • Publisher :Unknown
  • Release Date :2018-05-04
  • Total pages :382
  • ISBN : 1351091395
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Summary : Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods - such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.

Advanced Biotechnology

Advanced Biotechnology
  • Author : R C Dubey
  • Publisher :Unknown
  • Release Date :2014
  • Total pages :1161
  • ISBN : 812194290X
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Summary : The book embodies 22 chapters covering various important disciplines of biotechnology, such as cell biology, molecular biology, molecular genetics, biophysical methods, genomics and proteomics, metagenomics, enzyme technology, immune-technology, transgenic plants and animals, industrial microbiology and environmental biotechnology. The book is illustrative. It is written in a simple language

Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology
  • Author : Keith Wilson,John Walker
  • Publisher :Unknown
  • Release Date :2010-03-04
  • Total pages :744
  • ISBN : 0521516358
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Summary : Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Calculations for Molecular Biology and Biotechnology

Calculations for Molecular Biology and Biotechnology
  • Author : Frank H. Stephenson
  • Publisher :Unknown
  • Release Date :2010-07-30
  • Total pages :460
  • ISBN : 9780123756916
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Summary : Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: Updated and increased coverage of real time PCR and the mathematics used to measure gene expression More sample problems in every chapter for readers to practice concepts

Molecular Biology and Biotechnology

Molecular Biology and Biotechnology
  • Author : Robert A. Meyers
  • Publisher :Unknown
  • Release Date :1995-06-29
  • Total pages :1034
  • ISBN : 9780471186342
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Summary : Essential for the top of every desk and laboratory bench, this single volume reference is the first and last resource required to answer any question relating to molecular biology, biotechnology, or molecular medicine. This work contains the theory and techniques for understanding, modifying, manipulating, expressing, and synthesizing biological molecules, conformations, and aggregates. Over 250 carefully reviewed articles written by 378 eminent scientists in the field, provide detailed and readable coverage of today's most relevant topics including life processes at the molecular level, genetic disease diagnosis and genetic therapy, the theory and techniques for understanding, manipulating, and synthesizing biological molecules and their aggregates.

Analytical Techniques in Biochemistry and Molecular Biology

Analytical Techniques in Biochemistry and Molecular Biology
  • Author : Rajan Katoch
  • Publisher :Unknown
  • Release Date :2011-07-19
  • Total pages :441
  • ISBN : 9781441997852
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Summary : Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Advanced methods in plant breeding and biotechnology

Advanced methods in plant breeding and biotechnology
  • Author : David Ronald Murray
  • Publisher :Unknown
  • Release Date :1991
  • Total pages :365
  • ISBN : 9780851987064
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Summary :

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology
  • Author : Andreas Hofmann,Samuel Clokie
  • Publisher :Unknown
  • Release Date :2018-03-31
  • Total pages :962
  • ISBN : 1107162270
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Summary : A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.

An Introduction to Molecular Biotechnology

An Introduction to Molecular Biotechnology
  • Author : Michael Wink,Renate FitzRoy
  • Publisher :Unknown
  • Release Date :2006-10-02
  • Total pages :768
  • ISBN : 9783527314126
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Summary : On 800 pages this textbook provides students and professionals in life sciences, pharmacy and biochemistry with a very detailed introduction to molecular and cell biology, including standard techniques, key topics, and biotechnology in industry.

Molecular Biology and Biotechnology

Molecular Biology and Biotechnology
  • Author : Ralph Rapley,David Whitehouse
  • Publisher :Unknown
  • Release Date :2015-11-09
  • Total pages :229
  • ISBN : 1782625518
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Summary : One of the exciting aspects of being involved in the field of molecular biology is the ever-accelerating rate of progress, both in the development of new methodologies and the practical applications of these methodologies. This popular textbook has been completely revised and updated to provide a comprehensive overview and to reflect key developments in this rapidly expanding area. Chapters on the impact of molecular biology in the development of biotechnology have been fully updated and include the applications of molecular biology in the areas of diagnostics, biosensors and biomarkers, therapeutics, agricultural biotechnology and vaccines. The first six chapters deal with the technology used in current molecular biology and biotechnology. These primarily deal with core nucleic acid techniques, genomics, proteomics and recombinant protein production. Further chapters address major advances in the applications of molecular biotechnology. By presenting information in an easily assimilated form, this book makes an ideal undergraduate text. Molecular Biology and Biotechnology 6th Edition will be of particular interest to students of biology and chemistry, as well as to postgraduates and other scientific workers who need a sound introduction to this ever rapidly advancing and expanding area.

Basic Methods in Molecular Biology

Basic Methods in Molecular Biology
  • Author : Leonard Davis
  • Publisher :Unknown
  • Release Date :2012-12-02
  • Total pages :399
  • ISBN : 044460149X
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Summary : Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.