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Biomedical Optical Phase Microscopy and Nanoscopy

Biomedical Optical Phase Microscopy and Nanoscopy
  • Author : Natan T. Shaked,Zeev Zalevsky,Lisa L Satterwhite
  • Publisher :Unknown
  • Release Date :2012-12-31
  • Total pages :432
  • ISBN : 9780124158863
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Summary : Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications. Provides a comprehensive overview of the principles and techniques of optical phase microscopy and nanoscopy with biomedical applications. Tips/advice on building systems and working with advanced imaging biomedical techniques, including interpretation of phase images, and techniques for quantitative analysis based on phase microscopy. Interdisciplinary approach that combines optical engineering, nanotechnology, biology and medical aspects of this topic. Each chapter includes practical implementations and worked examples.

Label-Free Super-Resolution Microscopy

Label-Free Super-Resolution Microscopy
  • Author : Vasily Astratov
  • Publisher :Unknown
  • Release Date :2019-08-31
  • Total pages :487
  • ISBN : 9783030217228
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Summary : This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.

Biomedical Optical Imaging

Biomedical Optical Imaging
  • Author : James G. Fujimoto,Daniel Farkas
  • Publisher :Unknown
  • Release Date :2009-04-22
  • Total pages :440
  • ISBN : 9780190287788
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Summary : Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.

High Resolution Imaging in Microscopy and Ophthalmology

High Resolution Imaging in Microscopy and Ophthalmology
  • Author : Josef F. Bille
  • Publisher :Unknown
  • Release Date :2019-08-13
  • Total pages :407
  • ISBN : 9783030166380
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Summary : This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.

Techniques in Microscopy for Biomedical Applications

Techniques in Microscopy for Biomedical Applications
  • Author : Terje Dokland,Dietmar Werner Hutmacher,Mary Mah-Lee Ng,Jan-Thorsten Schantz
  • Publisher :Unknown
  • Release Date :2006-09-28
  • Total pages :420
  • ISBN : 9789813101951
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Summary : The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide those who require the use of microscopic for methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopic techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues. Microscopy has revolutionized our understanding of biological events. Within the last two decades, microscopic techniques have provided insights into the dynamics of biological processes that regulate such events. Biological discovery, to a large extent, depends on advances in imaging techniques and various microscopic techniques have emerged as central and indispensable tools in the biomedical sciences. The four authors bring with them extensive experiences spanning across disciplines such as Microbiology, Molecular and Cell Biology, Tissue Engineering, Biomedical and Regenerative Medicine and so forth, reinforcing the fact that microscopy has proven useful in countless investigations into the mysteries of life.

Field Guide to Microscopy

Field Guide to Microscopy
  • Author : Tomasz S. Tkaczyk
  • Publisher :Unknown
  • Release Date :2010
  • Total pages :138
  • ISBN : STANFORD:36105215503454
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Summary : This guide provides extensive coverage of microscopic imaging principles. After reviewing the main principles of image formation, diffraction, interference, and polarization used in microscopy, this guide describes the most widely applied microscope configurations and applications. It also covers major system components, including light sources, illumination layouts, microscope optics, and image detection electronics. This guide also provides a comprehensive overview of microscopy techniques, including bright field and dark field imaging, contrast enhancement methods (such as phase and amplitude contrast), DIC, polarization, and fluorescence microscopy. In addition, it describes scanning techniques (such as confocal and multiphoton imaging points); new trends in super-resolution methods (such as 4Pi microscopy, STED, STORM, and structured illumination); and, array microscopy, CARS, and SPIM.

Coherent Light Microscopy

Coherent Light Microscopy
  • Author : Pietro Ferraro,Adam Wax,Zeev Zalevsky
  • Publisher :Unknown
  • Release Date :2011-02-09
  • Total pages :372
  • ISBN : 3642158137
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Summary : This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.

Handbook of Biomedical Optics

Handbook of Biomedical Optics
  • Author : David A. Boas,Constantinos Pitris,Nimmi Ramanujam
  • Publisher :Unknown
  • Release Date :2016-04-19
  • Total pages :831
  • ISBN : 9781420090376
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Summary : Biomedical optics holds tremendous promise to deliver effective, safe, non- or minimally invasive diagnostics and targeted, customizable therapeutics. Handbook of Biomedical Optics provides an in-depth treatment of the field, including coverage of applications for biomedical research, diagnosis, and therapy. It introduces the theory and fundamental

Electrically Active Materials for Medical Devices

Electrically Active Materials for Medical Devices
  • Author : Syed A M Tofail,Joanna Bauer
  • Publisher :Unknown
  • Release Date :2016-08-04
  • Total pages :229
  • ISBN : 9781783269884
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Summary : Stress induced electrical charges, action potential and electret behavior of bone, muscles, skin and nerve cells have been known for some time. Electrically Active Materials for Medical Devices builds on this knowledge and encourages readers to understand and exploit electrical activity in biomaterials from native, derived, or completely synthetic origin, or a combination thereof. It presents data and insights from both historic and contemporary research that spans over six decades with a view to generate convergence of interdisciplinary knowledge and skills. Divided into four parts, this book first introduces the reader to a general overview of electrically active materials in biology and biomedical science and describes important concepts and pioneering discoveries. The second part discusses common types of materials that are known to generate electrical activity and lays the foundation for these materials for use in medical devices. The third part gives examples of where electrically active materials have been examined for device application. The final part looks for upcoming and emerging concepts, tools and methodologies that are expected to shape the future profile of this field of converging science. Written by specialists in their respective fields, it has been specifically targeted at a readership of professionals, graduate students and researchers in the fields of biomedical engineering, physics, chemistry biology and clinical medicine.

Global Health Informatics

Global Health Informatics
  • Author : Hamish S. F. Fraser
  • Publisher :Unknown
  • Release Date :2017-04-21
  • Total pages :464
  • ISBN : 9780262533201
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Summary : Key concepts, frameworks, examples, and lessons learned in designing and implementing health information and communication technology systems in the developing world.

Handbook of Analytical Techniques for Forensic Samples

Handbook of Analytical Techniques for Forensic Samples
  • Author : Chaudhery Mustansar Hussain,Deepak Rawtani,Gaurav Pandey,Maithri Tharmavaram
  • Publisher :Unknown
  • Release Date :2020-11-28
  • Total pages :452
  • ISBN : 9780128223017
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Summary : Handbook of Analytical Techniques for Forensic Samples: Current and Emerging Developments discusses in detail the current trends and latest analytical techniques and methods commonly employed in forensic analysis in order to ensure the proper facilitation of justice. This book is useful for readers who wish to stay updated on the latest trends in the forensic analysis of samples encountered at crime scenes. Technological advancements, such as biosensors, nanotechnology, and taggant technology have upped the level of analysis in forensic science. These emergent technologies, incorporated with existing analytical techniques, are leading to more precise, accurate, and specific examination of forensic samples. Lab-on-a-chip technology has also eased several kinds of on-site analyses done by investigating teams at different types of crime scenes. This book covers the evolution of forensic sample analysis as well as these emerging trends and new technologies. Includes an entire section of experimental exercises for self-teaching and key concept review Covers laboratory protocols used in forensic science laboratories for the analysis of various samples through different analytical techniques Condenses the many aspects of forensic analytical chemistry into a single resource with easy-to-understand language for everyone from students to practitioners

Holographic Materials and Optical Systems

Holographic Materials and Optical Systems
  • Author : Izabela Naydenova,Tsvetanka Babeva,Dimana Nazarova
  • Publisher :Unknown
  • Release Date :2017-03-22
  • Total pages :518
  • ISBN : 9789535130376
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Summary : Holographic Materials and Optical Systems covers recent research achievements in the areas of volume holographic optical elements and systems, development of functionalized holographic recording materials, and applications in holographic imaging and metrology. Designs of single and multiplexed volume holographic optical elements for laser beam shaping, combining, and redirection are covered, and their properties are studied theoretically and experimentally. The high impact of holography in imaging and metrology is demonstrated by applications spreading from thickness and surface measurements, through antenna metrology and analyzing high-density gradients in fluid mechanics to characterization of live objects in clinical diagnostics. Novel functionalized materials used in dynamic or permanent holographic recording cover photopolymers, photochromics, photo-thermo-refractive glasses, and hybrid organic-inorganic media.

Quantitative Phase Imaging

Quantitative Phase Imaging
  • Author : Gabriel Popescu,YongKeun Park
  • Publisher :Unknown
  • Release Date :2015
  • Total pages :229
  • ISBN : OCLC:1132085377
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Summary : 'Proceedings of SPIE' presents the original research papers presented at SPIE conferences and other high-quality conferences in the broad-ranging fields of optics and photonics. These books provide prompt access to the latest innovations in research and technology in their respective fields.

Understanding Biophotonics

Understanding Biophotonics
  • Author : Kevin Tsia
  • Publisher :Unknown
  • Release Date :2016-01-05
  • Total pages :766
  • ISBN : 9789814411783
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Summary : Biophotonics involves understanding how light interacts with biological matter, from molecules and cells, to tissues and even whole organisms. Light can be used to probe biomolecular events, such as gene expression and protein–protein interaction, with impressively high sensitivity and specificity. The spatial and temporal distribution of biochemical constituents can also be visualized with light and, thus, the corresponding physiological dynamics in living cells, tissues, and organisms in real time. Light can also be used to alter the properties and behaviors of biological matter, such as to damage cancerous cells by laser surgery or therapy, and manipulate the neuronal signaling in a brain network. Fueled by the innovations in photonic technologies in the past half century, biophotonics continues to play a ubiquitous role in revolutionizing basic life science studies as well as biomedical diagnostics and therapies. Advancements in biophotonics in the past few decades can be seen not only in biochemistry and cell/molecular biology, but also in numerous preclinical applications. Researchers around the world are searching for ways to bring biophotonic technologies into real clinical practices, particularly cellular and molecular optical imaging. Meanwhile, emerging technologies, such as laser nanosurgery and nanoplasmonics, have created new insights for understanding, monitoring, and even curing diseases on a molecular basis. This book presents the essential basics of optics and biophotonics to newcomers (senior undergraduates or postgraduate researchers) who are interested in this multidisciplinary research field. With stellar contributions from leading experts, the book highlights the major advancements in preclinical diagnostics using optical microscopy and spectroscopy, including multiphoton microscopy, super-resolution microscopy, and endomicroscopy. It also introduces a number of emerging techniques and toolsets for biophotonics applications, such as nanoplasmonics, microresonators for molecular detection, and subcellular optical nanosurgery.

Quantitative Phase Imaging of Cells and Tissues

Quantitative Phase Imaging of Cells and Tissues
  • Author : Gabriel Popescu
  • Publisher :Unknown
  • Release Date :2011-03-14
  • Total pages :400
  • ISBN : 9780071663434
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Summary : Cutting-edge quantitative phase imaging techniques and their applications Filled with unique, full-color images taken by advanced quantitative phase imaging (QPI), Quantitative Phase Imaging of Cells and Tissues thoroughly explores this innovative technology and its biomedical applications. An introductory background on optical imaging and traditional optical microscopy is included to illustrate concept development. The book explains how various visualization modalities can be obtained by numerical calculations. This authoritative resource reveals how to take full advantage of the unprecedented capabilities of QPI, such as rendering scattering properties of minute subcellular structures and nanoscale fluctuations in live cells. Coverage includes: Groundwork Spatiotemporal field correlations Image characteristics Light microscopy Holography Point scanning QPI methods Principles of full-field QPI Off-axis full-field methods Phase-shifting techniques Common-path methods White light techniques Fourier transform light scattering (FTLS) Current trends in QPI

Quantitative Biomedical Optics

Quantitative Biomedical Optics
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2021
  • Total pages :229
  • ISBN : 9780521876568
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Summary :

Nanoscopy and Multidimensional Optical Fluorescence Microscopy

Nanoscopy and Multidimensional Optical Fluorescence Microscopy
  • Author : Alberto Diaspro
  • Publisher :Unknown
  • Release Date :2010-04-26
  • Total pages :24
  • ISBN : STANFORD:36105215532651
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Summary : The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples. --

Nano-Optics

Nano-Optics
  • Author : Sabu Thomas,Yves Grohens,Guillaume Vignaud,Nandakumar Kalarikkal,Jemy James
  • Publisher :Unknown
  • Release Date :2020-07-06
  • Total pages :376
  • ISBN : 9780128183939
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Summary : Nano-Optics: Fundamentals, Experimental Methods, and Applications offers insights into the fundamentals and industrial applications of nanoscale light-emitting materials and their composites. This book serves as a reference, offering an overview of existing research, with a particular focus on industrial applications. Nano-optics is the branch of nanoscience and nanotechnology that deals with interaction of light with nanoscale objects. This book explores the materials, structure, manufacturing techniques, and industrial applications of nano-optics. The applications discussed include healthcare, communication, astronomy, and satellites. Explains the major manufacturing techniques for light-emitting nanoscale materials Discusses how nanoscale optical materials are being used in a range of industrial applications Assesses the challenges of using nano-optics in a mass-production context

Confocal Scanning Optical Microscopy and Related Imaging Systems

Confocal Scanning Optical Microscopy and Related Imaging Systems
  • Author : Gordon S. Kino,Timothy R. Corle
  • Publisher :Unknown
  • Release Date :1996-09-18
  • Total pages :335
  • ISBN : 008052978X
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Summary : This book provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers. The book concentrates mainly on two instruments: the Confocal Scanning Optical Microscope (CSOM), and the Optical Interference Microscope (OIM). A comprehensive discussion of the theory and design of the Near-Field Scanning Optical Microscope (NSOM) is also given. The text discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope, and the applications of these microscopes to phase imaging, biological imaging, and semiconductor inspection and metrology.A comprehensive theoretical discussion of the depth and transverse resolution is given with emphasis placed on the practical results of the theoretical calculations and how these can be used to help understand the operation of these microscopes. Provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers Explains many practical applications of scanning optical and interference microscopy in such diverse fields as biology and semiconductor metrology Discusses in theoretical terms the origin of the improved depth and transverse resolution of scanning optical and interference microscopes with emphasis on the practical results of the theoretical calculations Considers the practical aspects of building a confocal scanning or interference microscope and explores some of the design tradeoffs made for microscopes used in various applications Discusses the theory and design of near-field optical microscopes Explains phase imaging in the scanning optical and interference microscopes

Far-Field Optical Nanoscopy

Far-Field Optical Nanoscopy
  • Author : Philip Tinnefeld,Christian Eggeling,Stefan W. Hell
  • Publisher :Unknown
  • Release Date :2015-02-07
  • Total pages :335
  • ISBN : 9783662455470
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Summary : This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

Biomedical Optics

Biomedical Optics
  • Author : Lihong V. Wang,Hsin-i Wu
  • Publisher :Unknown
  • Release Date :2012-09-26
  • Total pages :376
  • ISBN : 9780470177006
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Summary : This entry-level textbook, covering the area of tissue optics, is based on the lecture notes for a graduate course (Bio-optical Imaging) that has been taught six times by the authors at Texas A&M University. After the fundamentals of photon transport in biological tissues are established, various optical imaging techniques for biological tissues are covered. The imaging modalities include ballistic imaging, quasi-ballistic imaging (optical coherence tomography), diffusion imaging, and ultrasound-aided hybrid imaging. The basic physics and engineering of each imaging technique are emphasized. A solutions manual is available for instructors; to obtain a copy please email the editorial department at [email protected]