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Essential Enzyme Kinetics

Essential Enzyme Kinetics
  • Author : Daniel L. Purich
  • Publisher :Unknown
  • Release Date :2020-06-01
  • Total pages :425
  • ISBN : 9780128218099
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Summary : Essential Enzyme Kinetics: A Textbook for Molecular Life Scientists describes the theoretical basis and best-practice approaches for using initial-rate, fast reaction, and kinetic isotope effect experiments to define enzyme catalysis. Because a detailed knowledge of enzyme transition-states is the main driver for the rational design of slow, tight-binding inhibitors destined to become tomorrow's small-molecule drugs, Essential Enzyme Kinetics is the must-have reference for chemists, biochemists, and pharmacologists intent on pursuing careers in Big Pharma. Given the interdisciplinary nature of contemporary drug development, this book provides a lucid short-course that will also benefit nonspecialists seeking to understand the scope and reach of modern enzyme kinetics. Provides practical information about how to work with enzymes and design experiments to identify new inhibitors or activators Includes detailed step-by-step derivations of rate equations, showing tried-and-true ways to confirm that the equations obtained are correct Arranged for use both as a desk reference (with over 200 equations and more than 400 key references) or as a textbook (with 8-10 problems/exercises at the end of each chapter)

Fundamentals of Enzyme Kinetics

Fundamentals of Enzyme Kinetics
  • Author : Athel Cornish-Bowden
  • Publisher :Unknown
  • Release Date :2014-05-20
  • Total pages :244
  • ISBN : 9781483161198
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Summary : Fundamentals of Enzyme Kinetics details the rate of reactions catalyzed by different enzymes and the effects of varying the conditions on them. The book includes the basic principles of chemical kinetics, especially the order of a reaction and its rate constraints. The text also gives an introduction to enzyme kinetics - the idea of an enzyme-substrate complex; the Michaelis-Menten equation; the steady state treatment; and the validity of its assumption. Practical considerations, the derivation of steady-state rate equations, inhibitors and activators, and two-substrate reactions are also explained. Problems after the end of each chapter have also been added, as well as their solutions at the end of the book, to test the readers' learning. The text is highly recommended for undergraduate students in biochemistry who wish to study about enzymes or focus completely on enzymology, as most of the mathematics used in this book, which have been explained in detail to remove most barriers of understanding, is elementary.

Kinetics of Enzyme Action

Kinetics of Enzyme Action
  • Author : Ross L. Stein
  • Publisher :Unknown
  • Release Date :2011-08-08
  • Total pages :320
  • ISBN : 1118084403
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Summary : Few scientists have the knowledge to perform the studies that are necessary to discover and characterize enzyme inhibitors, despite the vested interest the pharmaceutical industry has in this field. Beginning with the most basic principles pertaining to simple, one-substrate enzyme reactions and their inhibitors, and progressing to a thorough treatment of two-substrate enzymes, Kinetics of Enzyme Action: Essential Principles for Drug Hunters provides biochemists, medicinal chemists, and pharmaceutical scientists with numerous case study examples to outline the tools and techniques necessary to perform, understand, and interpret detailed kinetic studies for drug discovery.

Principles of Enzyme Kinetics

Principles of Enzyme Kinetics
  • Author : Athel Cornish-Bowden
  • Publisher :Unknown
  • Release Date :2014-05-20
  • Total pages :220
  • ISBN : 9781483164670
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Summary : Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity. Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable.

Enzyme Kinetics: Catalysis and Control

Enzyme Kinetics: Catalysis and Control
  • Author : Daniel L. Purich
  • Publisher :Unknown
  • Release Date :2010-06-16
  • Total pages :920
  • ISBN : 9780123809254
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Summary : Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions

Essentials of Enzymology

Essentials of Enzymology
  • Author : Rufus O. Okotore
  • Publisher :Unknown
  • Release Date :2015-03-13
  • Total pages :221
  • ISBN : 9781503527225
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Summary : Essentials of Enzymology provides concise information on an important area of the subject, Biochemistry. This may serve as course material for an advanced treatise in Enzymology designed for undergraduate science degree programs, especially B.Sc. (Hons ) Biochemistry and Chemistry. The book is in 12 chapters which has been divided into four distinct sections, thus (1) Basic enzyme chemistry and physiology. (2) Enzyme Kinetics, (3) Enzyme catalysis, Mechanisms and Regulation,(4)Applications of Enzymology. The Part 1 consists of four chapters that deal with the nature of enzymes- (history, properties and classifi cation), enzyme physiology; structure of enzymes, and analytical enzymology. Part 2 deals with Enzyme Kinetics which is treated in three chapters, and Part 3, made up of three chapters discuss Enzyme catalysis, mechanisms and regulation. Lastly, Part 4 consisting of two chapters deal with the applications of enzymology. Signifi cantly, the kinetics of enzyme catalyzed reactions in diverse experimental conditions, and also under various inhibition types are presented in a simple, mathematical lucid approach. The mechanisms of action for two atypical proteins-chymotrypsin and lysozyme, so also the identifi cation of active sites of enzymes by specifi c labels are discussed concisely. Lastly, the specifi c applications of enzymes in diagnostic medicine, industry, and also the new emerging area of enzyme biotechnology and enzyme bioinformatics are presented

Contemporary Enzyme Kinetics and Mechanism

Contemporary Enzyme Kinetics and Mechanism
  • Author : Daniel L. Purich
  • Publisher :Unknown
  • Release Date :1983-01-01
  • Total pages :566
  • ISBN : 9781483214221
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Summary : Selected Methods in Enzymology: Contemporary Enzyme Kinetics and Mechanism provides an introduction to enzyme kinetics and mechanism at an intermediate level. This book covers a variety of topics, including temperature effects in enzyme kinetics, cryoenzymology, substrate inhibition, enol intermediates enzymology, and heavy-atom isotope effects. Organized into 19 chapters, this book begins with an overview of derivation of rate equations as an integral part of the effective usage of kinetics as a tool. This text then examines the practical aspects of initial rate enzyme assay. Other chapters consider the basic procedures used in making decisions concerning kinetic mechanisms from initial-rate data. This book discusses as well the various aspects of both the theoretical background and the applications. The final chapter deals with the importance of achieving proficiency in formulating quantitative relationships describing enzyme behavior. This book is a valuable resource for students and research workers. Enzymologists and chemists will also find this book useful.

Enzyme Kinetics and Regulation

Enzyme Kinetics and Regulation
  • Author : Aaren Bennett
  • Publisher :Unknown
  • Release Date :2018-07-07
  • Total pages :328
  • ISBN : 9781839471766
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Summary : We live in the age of science-the human and numerous other living beings' genomes have been sequenced and we are beginning to understand the capacity of the metabolic machinery responsible for life on our planet. A huge number of new genes have been discovered, a significant number of these coding for enzymes of yet obscure capacity. Understanding the kinetic behavior of an enzyme provides clues to its possible physiological role. From a biotechnological perspective, knowledge of the reactant properties of an enzyme is required for the design of immobilized enzyme-based modern processes. Biotransformations are of key importance to the pharmaceutical and sustenance industries, and knowledge of the reactant properties of enzymes, essential. This book is tied in with understanding the principles of enzyme kinetics and knowing how to use mathematical models to describe the reactant capacity of an enzyme. Coverage of the material is in no way, shape or form exhaustive. There exist many books on enzyme kinetics that offer intensive, in-depth treatises of the subject. Intracellular and extracellular physiological cascades are regulated by initiation and hindrance of different enzymes involved in these pathways. Investigating and understanding the mechanism of enzyme hindrance has become the premise of development of pharmaceutical agents. Organically active regular and synthetic inhibitors have been developed and special emphasis has been placed on investigations that define their structure-work relationships in an effort to understand the inception of their natural properties. A powerful complement to the assessment of these agents is the preparation and subsequent examination of key fractional structures, deep-seated auxiliary adjustments and the corresponding unnatural enantiomers of characteristic items. We sincerely hope that this book will represent an element in the tool kit of graduate students in applied science and chemical and biochemical engineering and furthermore of undergraduate students with formal preparing in natural chemistry, biochemistry, thermodynamics and chemical reaction kinetics.

Enzyme Inhibitors and Activators

Enzyme Inhibitors and Activators
  • Author : Murat Şentürk
  • Publisher :Unknown
  • Release Date :2017-03-29
  • Total pages :268
  • ISBN : 9789535130574
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Summary : Over the recent years, medicinal chemistry has become responsible for explaining interactions of chemical molecule processes such that many scientists in the life sciences from agronomy to medicine are engaged in medicinal research. This book contains an overview focusing on the research area of enzyme inhibitor and activator, enzyme-catalyzed biotransformation, usage of microbial enzymes, enzymes associated with programmed cell death, natural products as potential enzyme inhibitors, protease inhibitors from plants in insect pest management, peptidases, and renin-angiotensin system. The book provides an overview on basic issues and some of the recent developments in medicinal science and technology. Especially, emphasis is devoted to both experimental and theoretical aspect of modern medicine. The primary target audience for the book includes students, researchers, chemists, molecular biologists, medical doctors, pharmacologists, and professionals who are interested in associated areas. The textbook is written by international scientists with expertise in biochemistry, enzymology, molecular biology, and genetics, many of which are active in biochemical and pharmacological research. I would like to acknowledge the authors for their contribution to the book. We hope that the textbook will enhance the knowledge of scientists in the complexities of some medical approaches; it will stimulate both professionals and students to dedicate part of their future research in understanding relevant mechanisms and applications of pharmacology.

Textbook of Veterinary Physiological Chemistry

Textbook of Veterinary Physiological Chemistry
  • Author : Larry R. Engelking
  • Publisher :Unknown
  • Release Date :2014-08-12
  • Total pages :786
  • ISBN : 9780123919106
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Summary : Bridging the gap between basic and clinical science concepts, the Textbook of Veterinary Physiological Chemistry, Third Edition offers broad coverage of biochemical principles for students and practitioners of veterinary medicine. The only recent biochemistry book written specifically for the veterinary field, this text covers cellular-level concepts related to whole-body physiologic processes in a reader-friendly, approachable manner. Each chapter is written in a succinct and concise style that includes an overview summary section, numerous illustrations for best comprehension of the subject matter, targeted learning objectives, and end of the chapter study questions to assess understanding. With new illustrations and an instructor website with updated PowerPoint images, the Textbook of Veterinary Physiological Chemistry, Third Edition, proves useful to students and lecturers from diverse educational backgrounds. Sectional exams and case studies, new to this edition, extend the breadth and depth of learning resources. Provides newly developed case studies that demonstrate practical application of concepts Presents comprehensive sectional exams for self-assessment Delivers instructor website with updated PowerPoint images and lecture slides to enhance teaching and learning Employs a succinct communication style in support of quick comprehension

Proteolytic Enzymes

Proteolytic Enzymes
  • Author : Erwin E. Sterchi,Walter Stöcker
  • Publisher :Unknown
  • Release Date :2013-11-11
  • Total pages :366
  • ISBN : 9783642598166
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Summary : Following an overview on proteolytic enzyme assays, this text covers procedures on how to investigate and study proteases. It describes the use of specific restriction proteases as well as inhibitors of proteases to prevent unwanted proteolysis.

Evaluation of Enzyme Inhibitors in Drug Discovery

Evaluation of Enzyme Inhibitors in Drug Discovery
  • Author : Robert A. Copeland
  • Publisher :Unknown
  • Release Date :2005-04-01
  • Total pages :384
  • ISBN : 9780471723264
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Summary :

Fundamentals of Enzyme Kinetics

Fundamentals of Enzyme Kinetics
  • Author : Athel Cornish-Bowden
  • Publisher :Unknown
  • Release Date :2014-05-20
  • Total pages :244
  • ISBN : 9781483161198
GET BOOK HERE

Summary : Fundamentals of Enzyme Kinetics details the rate of reactions catalyzed by different enzymes and the effects of varying the conditions on them. The book includes the basic principles of chemical kinetics, especially the order of a reaction and its rate constraints. The text also gives an introduction to enzyme kinetics - the idea of an enzyme-substrate complex; the Michaelis-Menten equation; the steady state treatment; and the validity of its assumption. Practical considerations, the derivation of steady-state rate equations, inhibitors and activators, and two-substrate reactions are also explained. Problems after the end of each chapter have also been added, as well as their solutions at the end of the book, to test the readers' learning. The text is highly recommended for undergraduate students in biochemistry who wish to study about enzymes or focus completely on enzymology, as most of the mathematics used in this book, which have been explained in detail to remove most barriers of understanding, is elementary.

Enzyme Kinetics and Mechanism

Enzyme Kinetics and Mechanism
  • Author : Paul F. Cook,W. W. Cleland
  • Publisher :Unknown
  • Release Date :2007-03-06
  • Total pages :416
  • ISBN : 9781136844270
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Summary : Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.

Kinetics of Enzyme-Modifier Interactions

Kinetics of Enzyme-Modifier Interactions
  • Author : Antonio Baici
  • Publisher :Unknown
  • Release Date :2015-06-24
  • Total pages :489
  • ISBN : 9783709114025
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Summary : The kinetic mechanisms by which enzymes interact with inhibitors and activators, collectively called modifiers, are scrutinized and ranked taxonomically into autonomous species in a way similar to that used in the biological classification of plants and animals. The systematization of the mechanisms is based on two fundamental characters: the allosteric linkage between substrate and modifier and the factor by which a modifier affects the catalytic constant of the enzyme. Combinations of the physically significant states of these two characters in an ancestor-descendant-like fashion reveal the existence of seventeen modes of interaction that cover the needs of total, partial and fine-tuning modulation of enzyme activity. These interactions comprise five linear and five hyperbolic inhibition mechanisms, five nonessential activation mechanisms and two hybrid species that manifest either hyperbolic inhibition or nonessential activation characteristics depending on substrate concentration. Five essential activation mechanisms, which are taxonomically independent of the mentioned basic species, complete the inventory of enzyme modifiers. Often masked under conventional umbrella terms or treated as anomalous cases, all seventeen basic inhibition and nonessential activation mechanisms are represented in the biochemical and pharmacological literature of this and the past century, either in the form of rapid or slow-onset reversible interactions, or as irreversible modification processes. The full potential of enzyme inhibitors and activators can only be appreciated after elucidating the details of their kinetic mechanisms of action exploring the entire range of physiologically significant reactant concentrations. This book highlights the wide spectrum of allosteric enzyme modification in physiological occurrences as well as in pharmacological and biotechnological applications that embrace simple and multiple enzyme-modifier interactions. The reader is guided in the journey through this still partly uncharted territory with the aid of mechanistically-oriented criteria aimed at showing the logical way towards the identification of a particular mechanism.

ENZYMES: Catalysis, Kinetics and Mechanisms

ENZYMES: Catalysis, Kinetics and Mechanisms
  • Author : N.S. Punekar
  • Publisher :Unknown
  • Release Date :2018-11-11
  • Total pages :562
  • ISBN : 9789811307850
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Summary : This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to plan enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.

Essentials of Biochemistry

Essentials of Biochemistry
  • Author : Herbert J. Fromm,Mark Hargrove
  • Publisher :Unknown
  • Release Date :2012-01-05
  • Total pages :364
  • ISBN : 9783642196232
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Summary : This textbook, Essentials of Biochemistry is aimed at chemistry and biochemistry undergraduate students and first year biochemistry graduate students. It incorporates the lectures of the authors given to students with a strong chemistry background. An emphasis is placed on metabolism and reaction mechanisms and how they are studied. As the title of the book implies, the text lays the basis for an understanding of the fundamentals of biochemistry.

Current Advances in the Research of RNA Regulatory Enzymes

Current Advances in the Research of RNA Regulatory Enzymes
  • Author : Akio Kanai,Tohru Yoshihisa
  • Publisher :Unknown
  • Release Date :2020-01-02
  • Total pages :229
  • ISBN : 9782889632152
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Summary :

Enzymes

Enzymes
  • Author : Robert A. Copeland
  • Publisher :Unknown
  • Release Date :2004-04-07
  • Total pages :416
  • ISBN : 9780471461852
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Summary : Fully updated and expanded-a solid foundation for understandingexperimental enzymology. This practical, up-to-date survey is designed for a broadspectrum of biological and chemical scientists who are beginning todelve into modern enzymology. Enzymes, Second Editionexplains the structural complexities of proteins and enzymes andthe mechanisms by which enzymes perform their catalytic functions.The book provides illustrative examples from the contemporaryliterature to guide the reader through concepts and data analysisprocedures. Clear, well-written descriptions simplify the complexmathematical treatment of enzyme kinetic data, and numerouscitations at the end of each chapter enable the reader to accessthe primary literature and more in-depth treatments of specifictopics. This Second Edition of Enzymes: A Practical Introductionto Structure, Mechanism, and Data Analysis features refinedand expanded coverage of many concepts, while retaining theintroductory nature of the book. Important new featuresinclude: A new chapter on protein-ligand binding equilibria Expanded coverage of chemical mechanisms in enzyme catalysisand experimental measurements of enzyme activity Updated and refined discussions of enzyme inhibitors andmultiple substrate reactions Coverage of current practical applications to the study ofenzymology Supplemented with appendices providing contact information forsuppliers of reagents and equipment for enzyme studies, as well asa survey of useful Internet sites and computer software forenzymatic data analysis, Enzymes, Second Edition isthe ultimate practical guide for scientists and students inbiochemical, pharmaceutical, biotechnical, medicinal, andagricultural/food-related research.

Enzyme Kinetics in Drug Metabolism

Enzyme Kinetics in Drug Metabolism
  • Author : Swati Nagar,Upendra Argikar,Donald Tweedie
  • Publisher :Unknown
  • Release Date :2014-02-13
  • Total pages :538
  • ISBN : 1627037578
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Summary : Drug metabolism and transport are very important facets within the discipline of pharmaceutical sciences, with enzyme kinetic concepts utilized regularly in characterizing and modeling the disposition and elimination of drugs. Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications focuses on very practical aspects of applying kinetic principles to drug metabolizing enzymes and transporters. Divided into five convenient sections, topics include the fundamental principles of enzyme kinetics, the kinetics of oxidative and conjugative drug metabolizing enzymes and drug transporters, modeling approaches for both drug metabolizing enzymes and transporters including novel systems biology approaches, understanding of variability both experimental and interindividual (pharmacogenomic), and case studies that provide real life examples of applying these principles. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics especially suitable for the novice, in some cases step-by-step, readily reproducible protocols, and insights to help with troubleshooting and avoiding known pitfalls with extensive cross referencing to assist in learning. Authoritative and easily accessible, Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications serves as a very practical teaching tool for novice, non-mathematically trained scientists interested in these fundamental concepts and as an aid for their supervisors in teaching these principles.

Evaluation of Enzyme Inhibitors in Drug Discovery

Evaluation of Enzyme Inhibitors in Drug Discovery
  • Author : Robert A. Copeland
  • Publisher :Unknown
  • Release Date :2013-01-31
  • Total pages :572
  • ISBN : 9781118540282
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Summary : Offers essential guidance for discovering and optimizingnovel drug therapies Using detailed examples, Evaluation of Enzyme Inhibitors inDrug Discovery equips researchers with the tools needed toapply the science of enzymology and biochemistry to the discovery,optimization, and preclinical development of drugs that work byinhibiting specific enzyme targets. Readers will applaud this bookfor its clear and practical presentations, including its expertadvice on best practices to follow and pitfalls to avoid. This Second Edition brings the book thoroughly up to datewith the latest research findings and practices. Updates exploreadditional forms of enzyme inhibition and special treatments forenzymes that act on macromolecular substrates. Readers will alsofind new discussions detailing the development and application ofthe concept of drug-target residence time. Evaluation of Enzyme Inhibitors in Drug Discovery beginsby explaining why enzymes are such important drug targets and thenexamines enzyme reaction mechanisms. The book covers: Reversible modes of inhibitor interactions with enzymes Assay considerations for compound library screening Lead optimization and structure-activity relationships forreversible inhibitors Slow binding and tight binding inhibitors Drug-target residence time Irreversible enzyme inactivators The book ends with a new chapter exploring the application ofquantitative biochemical principles to the pharmacologic evaluationof drug candidates during lead optimization and preclinicaldevelopment. The Second Edition of Evaluation of Enzyme Inhibitorsin Drug Discovery continues to offer a treatment of enzymologyapplied to drug discovery that is quantitative and mathematicallyrigorous. At the same time, the clear and simple presentationsdemystify the complex science of enzymology, making the bookaccessible to many fields— from pharmacology to medicinalchemistry to biophysics to clinical medicine.