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Experiments in the Purification and Characterization of Enzymes

Experiments in the Purification and Characterization of Enzymes
  • Author : Thomas E. Crowley,Jack Kyte
  • Publisher :Unknown
  • Release Date :2014-01-11
  • Total pages :266
  • ISBN : 9780124095939
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Summary : Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Offers project lab formats for students that closely simulate original research projects Provides instructional guidance for students to design their own experiments Includes advanced analytical techniques Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH Includes access to a website with additional resources for instructors

Biochemistry in the Lab

Biochemistry in the Lab
  • Author : Benjamin F. Lasseter
  • Publisher :Unknown
  • Release Date :2019-09-30
  • Total pages :168
  • ISBN : 9780429957383
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Summary : Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps. Key Features: Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience Includes chemical biology as its foundation, teaching readers experimental methods specific to the field Provides instructor experiments that are easy to prepare and execute, at comparatively low cost Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry Is written by an expert in the field This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.

The FASEB Journal

The FASEB Journal
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1991
  • Total pages :229
  • ISBN : UCSD:31822009505751
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Summary :

Clinical and Experimental Hypertension

Clinical and Experimental Hypertension
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1991
  • Total pages :229
  • ISBN : NWU:35558002569230
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Summary :

Amino-acid, Peptide & Protein Abstracts

Amino-acid, Peptide & Protein Abstracts
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1978
  • Total pages :229
  • ISBN : UCAL:B4435361
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Summary :

Hatching Enzyme in the Sea Urchin Strongylocentrotus Purpuratus: Purification, Characterization, and Time of Synthesis

Hatching Enzyme in the Sea Urchin Strongylocentrotus Purpuratus: Purification, Characterization, and Time of Synthesis
  • Author : Benjamin Frank Edwards
  • Publisher :Unknown
  • Release Date :1978
  • Total pages :232
  • ISBN : UCAL:X14092
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Summary :

Bibliography of Agriculture with Subject Index

Bibliography of Agriculture with Subject Index
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2000
  • Total pages :229
  • ISBN : UOM:39015055285434
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Summary :

Bibliography of Agriculture

Bibliography of Agriculture
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1990-08
  • Total pages :229
  • ISBN : PSU:000057553788
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Summary :

Purification, Characterization, and Localization of Staphylococcal Acid Phosphatase

Purification, Characterization, and Localization of Staphylococcal Acid Phosphatase
  • Author : Floyd Joseph Malveaux
  • Publisher :Unknown
  • Release Date :1968
  • Total pages :222
  • ISBN : MSU:31293015914546
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Summary :

Purification, Characterization and Analysis of the Regulation of Three Exocellular Proteinases from Ṯṟi̲c̲ẖo̲p̲ẖy̲ṯo̲ṉ ̲ṟu̲ḇṟu̲m̲

Purification, Characterization and Analysis of the Regulation of Three Exocellular Proteinases from Ṯṟi̲c̲ẖo̲p̲ẖy̲ṯo̲ṉ ̲ṟu̲ḇṟu̲m̲
  • Author : Gerard L. Apodaca
  • Publisher :Unknown
  • Release Date :1989
  • Total pages :240
  • ISBN : UCAL:X49722
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Summary :

Federation Proceedings

Federation Proceedings
  • Author : Federation of American Societies for Experimental Biology
  • Publisher :Unknown
  • Release Date :1987
  • Total pages :229
  • ISBN : UVA:X001415706
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Summary :

Introductory Experiments on Biomolecules and their Interactions

Introductory Experiments on Biomolecules and their Interactions
  • Author : Robert K. Delong,Qiongqiong Zhou
  • Publisher :Unknown
  • Release Date :2015-03-06
  • Total pages :126
  • ISBN : 9780128010129
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Summary : Introductory Experiments on Biomolecules and their Interactions provides a novel approach to teaching biomolecules in the lab. While featuring the requisite fundamentals, it also captures the author’s experience in industry, thus providing unique, up-to-date experiments which take the learning experience one-step further. The text parallels lectures using a standard biochemistry undergraduate text. Unlike most current lab manuals available in the market which simply emphasize an introduction of techniques, this lab manual provides students with opportunities to demonstrate and prove the knowledge and theories they learn from class. Features quantitative analysis of RNA degradation by RNase Contains problem sets, calculations, and references for each lab fully immersing students in the learning process Includes instruction on how to maintain a lab notebook and write a formal lab report Provides hands-on engagement with the four major types of biomolecules and “real-life and better applied examples of molecular interactions

Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals

Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals
  • Author : Brian H. Davison
  • Publisher :Unknown
  • Release Date :2005-04
  • Total pages :1166
  • ISBN : 9781588296979
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Summary : Annotation State-of-the-art research by leading experts ## Advanced feedstock production and processing ## Enzyme and microbial biocatalysis ## Bioprocess research and development ## Commercialization of biobased products.

Journal of Experimental Biology

Journal of Experimental Biology
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2001
  • Total pages :229
  • ISBN : NWU:35558004577322
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Summary :

Xylanolytic Enzymes

Xylanolytic Enzymes
  • Author : Pratima Bajpai
  • Publisher :Unknown
  • Release Date :2014-02-03
  • Total pages :120
  • ISBN : 9780128010662
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Summary : Xylanolytic Enzymes describes the enzyme structure and its interaction with plant cell walls, the properties and production of different enzymes and their application, and the knowledge gathered on the hydrolysis mechanism of hemicellulose. The knowledge gathered about the hydrolysis mechanism of the hemicelluloses, especially xylans, has greatly promoted the rapid application of these enzymes in new areas. Recently there has been much industrial interest in xylan and its hydrolytic enzymatic complex, as a supplement and for the manufacturing of food, drinks, textiles, pulps and paper, and ethanol; and in xylitol production as a fermentation substrate for the production of enzymes. This book describes xylan as a major component of plant hemicelluloses. Presents a thorough overview of all aspects of xylanolytic enzymes Gives up-to-date authoritative information and cites pertinent research Includes studies on xylanase regulation and synergistic action between multiple forms of xylanase

New Scientist

New Scientist
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1979
  • Total pages :229
  • ISBN : UCSD:31822015707771
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Summary :

Structure in Protein Chemistry

Structure in Protein Chemistry
  • Author : Jack Kyte
  • Publisher :Unknown
  • Release Date :2006-11-01
  • Total pages :832
  • ISBN : 9781136843501
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Summary : The second edition of Structure in Protein Chemistry showcases the latest developments and innovations in the field of protein structure analysis and prediction. The book begins by explaining how proteins are purified and describes methods for elucidating their sequences of amino acids and defining their posttranslational modifications. Comprehensive explanations of crystallography and of noncovalent forces-ionic interactions, hydrogen bonding, and the hydrophobic effect-act as a prelude to an exhaustive description of the atomic details of the structures of proteins. The resulting understanding of protein molecular structure forms the basis for discussions of the evolution of proteins, the symmetry of the oligomeric associations that produce them, and the chemical, mathematical, and physical basis of the techniques used to study their structures. The latter include image reconstruction, nuclear magnetic resonance spectroscopy, proton exchange, optical spectroscopy, electrophoresis, covalent cross-linking, chemical modification, immunochemistry, hydrodynamics, and the scattering of light, X-radiation, and neutrons. These procedures are applied to study the folding of polypeptides and the assembly of oligomers. Biological membranes and their proteins are also discussed. Structure in Protein Chemistry, Second Edition, bridges the gap between introductory biophysical chemistry courses and research literature. It serves as a comprehensive textbook for advanced undergraduates and graduate students in biochemistry, biophysics, and structural and molecular biology. Professionals engaged in chemical, biochemical, and molecular biological research will find it a useful reference.

Principles and Reactions of Protein Extraction, Purification, and Characterization

Principles and Reactions of Protein Extraction, Purification, and Characterization
  • Author : Hafiz Ahmed,Hafiz Ahmed PhD
  • Publisher :Unknown
  • Release Date :2017-07-27
  • Total pages :408
  • ISBN : 0203507436
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Summary : Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different

Dissertation Abstracts International

Dissertation Abstracts International
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2001
  • Total pages :229
  • ISBN : STANFORD:36105110578692
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Summary :

PARTIAL PURIFICATION, CHARACTERIZATION, AND BINDING STUDY OF A PEROXIDASE FROM HUMAN PLACENTA.

PARTIAL PURIFICATION, CHARACTERIZATION, AND BINDING STUDY OF A PEROXIDASE FROM HUMAN PLACENTA.
  • Author : JOHN LINCOLN NELSON
  • Publisher :Unknown
  • Release Date :1988
  • Total pages :106
  • ISBN : UOM:39015025394118
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Summary : the oxidation of certain carcinogens to reactive intermediates capable of binding to biological macromolecules, suggesting a potential role in the bioactivation of fetotoxicants.

Enzyme Inhibitors and Activators

Enzyme Inhibitors and Activators
  • Author : Murat Şentürk
  • Publisher :Unknown
  • Release Date :2017-03-29
  • Total pages :268
  • ISBN : 9789535130574
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Summary : Over the recent years, medicinal chemistry has become responsible for explaining interactions of chemical molecule processes such that many scientists in the life sciences from agronomy to medicine are engaged in medicinal research. This book contains an overview focusing on the research area of enzyme inhibitor and activator, enzyme-catalyzed biotransformation, usage of microbial enzymes, enzymes associated with programmed cell death, natural products as potential enzyme inhibitors, protease inhibitors from plants in insect pest management, peptidases, and renin-angiotensin system. The book provides an overview on basic issues and some of the recent developments in medicinal science and technology. Especially, emphasis is devoted to both experimental and theoretical aspect of modern medicine. The primary target audience for the book includes students, researchers, chemists, molecular biologists, medical doctors, pharmacologists, and professionals who are interested in associated areas. The textbook is written by international scientists with expertise in biochemistry, enzymology, molecular biology, and genetics, many of which are active in biochemical and pharmacological research. I would like to acknowledge the authors for their contribution to the book. We hope that the textbook will enhance the knowledge of scientists in the complexities of some medical approaches; it will stimulate both professionals and students to dedicate part of their future research in understanding relevant mechanisms and applications of pharmacology.