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Fed-Batch Fermentation

Fed-Batch Fermentation
  • Author : G G Moulton
  • Publisher :Unknown
  • Release Date :2014-10-16
  • Total pages :188
  • ISBN : 9781908818331
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Summary : Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies. This process can be used as a teaching tool for the inexperienced fermentation student or researcher in the fields of bioprocessing and bioreactors. It is an important segue from E. coli shake flask cultures to bioreactor The fed-batch fermentation is designed to be accomplished in a single day with the preparation work being done on the day prior The fed-batch fermentation described in this book is a robust process and can be easily scaled for CMO production of protein product

Fed-Batch Cultures

Fed-Batch Cultures
  • Author : Henry C. Lim,Hwa Sung Shin
  • Publisher :Unknown
  • Release Date :2013-04-22
  • Total pages :229
  • ISBN : 9781107328129
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Summary : Many, if not most, industrially important fermentation and bioreactor operations are carried out in fed-batch mode, producing a wide variety of products. In spite of this, there is no single book that deals with fed-batch operations. This is the first book that presents all the necessary background material regarding the 'what, why and how' of optimal and sub-optimal fed-batch operations. Numerous examples are provided to illustrate the application of optimal fed-batch cultures. This unique book, by world experts with decades of research and industrial experience, is a must for researchers and industrial practitioners of fed-batch processes (modeling, control and optimization) in biotechnology, fermentation, food, pharmaceuticals and waste treatment industries.

Bioprocess Engineering Principles

Bioprocess Engineering Principles
  • Author : Pauline M. Doran
  • Publisher :Unknown
  • Release Date :1995-04-03
  • Total pages :439
  • ISBN : 0080528120
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Summary : The emergence and refinement of techniques in molecular biology has changed our perceptions of medicine, agriculture and environmental management. Scientific breakthroughs in gene expression, protein engineering and cell fusion are being translated by a strengthening biotechnology industry into revolutionary new products and services. Many a student has been enticed by the promise of biotechnology and the excitement of being near the cutting edge of scientific advancement. However, graduates trained in molecular biology and cell manipulation soon realise that these techniques are only part of the picture. Reaping the full benefits of biotechnology requires manufacturing capability involving the large-scale processing of biological material. Increasingly, biotechnologists are being employed by companies to work in co-operation with chemical engineers to achieve pragmatic commercial goals. For many years aspects of biochemistry and molecular genetics have been included in chemical engineering curricula, yet there has been little attempt until recently to teach aspects of engineering applicable to process design to biotechnologists. This textbook is the first to present the principles of bioprocess engineering in a way that is accessible to biological scientists. Other texts on bioprocess engineering currently available assume that the reader already has engineering training. On the other hand, chemical engineering textbooks do not consider examples from bioprocessing, and are written almost exclusively with the petroleum and chemical industries in mind. This publication explains process analysis from an engineering point of view, but refers exclusively to the treatment of biological systems. Over 170 problems and worked examples encompass a wide range of applications, including recombinant cells, plant and animal cell cultures, immobilised catalysts as well as traditional fermentation systems. * * First book to present the principles of bioprocess engineering in a way that is accessible to biological scientists * Explains process analysis from an engineering point of view, but uses worked examples relating to biological systems * Comprehensive, single-authored * 170 problems and worked examples encompass a wide range of applications, involving recombinant plant and animal cell cultures, immobilized catalysts, and traditional fermentation systems * 13 chapters, organized according to engineering sub-disciplines, are groupled in four sections - Introduction, Material and Energy Balances, Physical Processes, and Reactions and Reactors * Each chapter includes a set of problems and exercises for the student, key references, and a list of suggestions for further reading * Includes useful appendices, detailing conversion factors, physical and chemical property data, steam tables, mathematical rules, and a list of symbols used * Suitable for course adoption - follows closely curricula used on most bioprocessing and process biotechnology courses at senior undergraduate and graduate levels.

A Cell Engineering Approach to Enzyme-based Fed-batch Fermentation

A Cell Engineering Approach to Enzyme-based Fed-batch Fermentation
  • Author : Michael Sibley
  • Publisher :Unknown
  • Release Date :2018
  • Total pages :229
  • ISBN : OCLC:1103954164
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Summary :

Control of the Quasi-steady State in Fed-batch Fermentation

Control of the Quasi-steady State in Fed-batch Fermentation
  • Author : Nicolas Kalogerakis
  • Publisher :Unknown
  • Release Date :1979
  • Total pages :282
  • ISBN : OCLC:61534786
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Summary :

Fermentation Microbiology and Biotechnology, Fourth Edition

Fermentation Microbiology and Biotechnology, Fourth Edition
  • Author : E. M. T. El-Mansi,Jens Nielsen,David Mousdale,Ross P. Carlson
  • Publisher :Unknown
  • Release Date :2018-12-17
  • Total pages :420
  • ISBN : 9780429015953
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Summary : Fermentation Microbiology and Biotechnology, 4th Edition explores and illustrates the broad array of metabolic pathways employed for the production of primary and secondary metabolites, as well as biopharmaceuticals. This updated and expanded edition addresses the whole spectrum of fermentation biotechnology, from fermentation kinetics and dynamics to protein and co-factor engineering. It also sheds light on the new strategies employed by industrialist for increasing tolerance and endurance of microorganisms to the accumulation of toxic wastes in microbial-cell factories. The new edition builds upon the fine pedigree of its earlier predecessors and extends the spectrum of the book to reflect the multidisciplinary and buoyant nature of this subject area. Key Features Covers the whole spectrum of the field from fermentation kinetics to control of fermentation and protein engineering. Includes case studies specifically designed to illustrate industrial applications and current state-of-the-art technologies. Presents the contributions of eminent international academics and industrial experts. Offers new chapters addressing: The prospects and the role of bio-fuels refineries, Control of metabolic efflux to product formation in microbial-cell factories and Improving tolerance of microorganisms to toxic byproduct accumulation in the fermentation vessel.

Increasing Final Concentration of L-lactic Acid Via Fed-batch Fermentation by Bacillus Aerolacticus BC-001

Increasing Final Concentration of L-lactic Acid Via Fed-batch Fermentation by Bacillus Aerolacticus BC-001
  • Author : Anunyaporn Wutthimongkholchai
  • Publisher :Unknown
  • Release Date :2016
  • Total pages :229
  • ISBN : OCLC:1102943615
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Summary :

Biochemical Engineering and Biotechnology

Biochemical Engineering and Biotechnology
  • Author : Ghasem Najafpour
  • Publisher :Unknown
  • Release Date :2015-02-24
  • Total pages :668
  • ISBN : 9780444633774
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Summary : Biochemical Engineering and Biotechnology, 2nd Edition, outlines the principles of biochemical processes and explains their use in the manufacturing of every day products. The author uses a diirect approach that should be very useful for students in following the concepts and practical applications. This book is unique in having many solved problems, case studies, examples and demonstrations of detailed experiments, with simple design equations and required calculations. Covers major concepts of biochemical engineering and biotechnology, including applications in bioprocesses, fermentation technologies, enzymatic processes, and membrane separations, amongst others Accessible to chemical engineering students who need to both learn, and apply, biological knowledge in engineering principals Includes solved problems, examples, and demonstrations of detailed experiments with simple design equations and all required calculations Offers many graphs that present actual experimental data, figures, and tables, along with explanations

Biopolyesters

Biopolyesters
  • Author : Wolfgang Babel,Alexander Steinbüchel
  • Publisher :Unknown
  • Release Date :2003-07-01
  • Total pages :342
  • ISBN : 9783540400219
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Summary : Living systems synthesize seven different classes of polymers. They provide structure and form for cells and organisms, function as catalysts and energy storage and carry the genetic information. All these polymers possess technically interesting properties. Some of these biopolymers are already used commercially. This special volume of Advances in Biochemical Engineering/Biotechnology comprises 10 chapters. It gives an overview of the water insoluble biopolyesters, in particular of the microbially synthesized poly-hydroxyalkanoate (PHA) family. It reports the state of the art of metabolism, regulation and genetic background, the latest advances made in genetic optimization of bacteria, "construction" of transgenic plants and in vitro synthesis by means of purified enzymes. Furthermore, it describes relevant technologies and evaluates perspectives concerning increasing the economic viability and competitiveness of PHA and discusses applications in medicine, packaging, food and other fields.

Advanced Instrumentation, Data Interpretation, and Control of Biotechnological Processes

Advanced Instrumentation, Data Interpretation, and Control of Biotechnological Processes
  • Author : J.F. van Impe,P.A. Vanrolleghem,D.M. Iserentant
  • Publisher :Unknown
  • Release Date :2013-06-29
  • Total pages :464
  • ISBN : 9789401591119
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Summary : This book is the outgrowth of the COMETT II Course on Advanced Instru mentation, Data Interpretation, and Control of Biotechnological Processes organized by the Katholieke Universiteit Leuven and the Universiteit Gent, and held at Gent, Belgium, October 1994. The editors of the present volume were very fortunate to find all invited speakers prepared to write state-of-the-art expositions based on their lec tures. Special thanks are due to all of them. The result is an account of recent advances in instrumentation, data interpretation, and model based op timization and control of bioprocesses. For anyone interested in this emerg ing field, this text is of value and provides comprehensive reviews as well as new and important trends and directions for the future, motivated and illustrated by a wealth of applications. The typesetting of all this material represented a tremendous amount of work. I am most grateful to my wife, Myriam Uyttendaele, and to Kurt Gheys, who did most of the proof-reading. Their efforts have increased a lot the uniformity in style and presentation of the different manuscripts. Many thanks also to the co-editors, for their continued support. Kluwer Academic Publishers is gratefully acknowledged for publishing this book, thus contributing to the transfer of the latest research results into large scale industrial applications.

Biomass Now

Biomass Now
  • Author : Miodrag Darko Matovic
  • Publisher :Unknown
  • Release Date :2013-04-30
  • Total pages :554
  • ISBN : 9789535111054
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Summary : This two-volume book on biomass is a reflection of the increase in biomass related research and applications, driven by overall higher interest in sustainable energy and food sources, by increased awareness of potentials and pitfalls of using biomass for energy, by the concerns for food supply and by multitude of potential biomass uses as a source material in organic chemistry, bringing in the concept of bio-refinery. It reflects the trend in broadening of biomass related research and an increased focus on second-generation bio-fuels. Its total of 40 chapters spans over diverse areas of biomass research, grouped into 9 themes.

Gene Expression Systems

Gene Expression Systems
  • Author : Joseph M. Fernandez,James P. Hoeffler
  • Publisher :Unknown
  • Release Date :1998-12-21
  • Total pages :480
  • ISBN : 9780080532356
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Summary : Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Provides detailed information on expression systems Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs Includes timelines and examples Compares pros and cons of each method

Industrial Biotechnology

Industrial Biotechnology
  • Author : Christoph Wittmann
  • Publisher :Unknown
  • Release Date :2017-03-06
  • Total pages :640
  • ISBN : 9783527341818
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Summary : The latest volume in the Advanced Biotechnology series provides an overview of the main product classes and platform chemicals produced by biotechnological processes today, with applications in the food, healthcare and fine chemical industries. Alongside the production of drugs and flavors as well as amino acids, bio-based monomers and polymers and biofuels, basic insights are also given as to the biotechnological processes yielding such products and how large-scale production may be enabled and improved. Of interest to biotechnologists, bio and chemical engineers, as well as those working in the biotechnological, chemical, and food industries.

Pichia Protocols

Pichia Protocols
  • Author : James M Cregg
  • Publisher :Unknown
  • Release Date :2007-08-08
  • Total pages :268
  • ISBN : 9781588294296
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Summary : This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.

Fermentation Processes

Fermentation Processes
  • Author : Angela Jozala
  • Publisher :Unknown
  • Release Date :2017-02-08
  • Total pages :312
  • ISBN : 9789535129271
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Summary : Fermentation is a theme widely useful for food, feed and biofuel production. Indeed each of these areas, food industry, animal nutrition and energy production, has considerable presence in the global market. Fermentation process also has relevant applications on medical and pharmaceutical areas, such as antibiotics production. The present book, Fermentation Processes, reflects that wide value of fermentation in related areas. It holds a total of 14 chapters over diverse areas of fermentation research.

Biotechnology of Microbial Enzymes

Biotechnology of Microbial Enzymes
  • Author : Goutam Brahmachari
  • Publisher :Unknown
  • Release Date :2016-07-21
  • Total pages :632
  • ISBN : 9780128037461
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Summary : Biotechnology of Microbial Enzymes: Production, Biocatalysis and Industrial Applications provides a complete survey of the latest innovations on microbial enzymes, highlighting biotechnological advances in their production and purification along with information on successful applications as biocatalysts in several chemical and industrial processes under mild and green conditions. Applications of microbial enzymes in food, feed, and pharmaceutical industries are given particular emphasis. The application of recombinant DNA technology within industrial fermentation and the production of enzymes over the last 20 years have produced a host of useful chemical and biochemical substances. The power of these technologies results in novel transformations, better enzymes, a wide variety of applications, and the unprecedented development of biocatalysts through the ongoing integration of molecular biology methodology, all of which is covered insightfully and in-depth within the book. Features research on microbial enzymes from basic science through application in multiple industry sectors for a comprehensive approach Includes information on metabolic pathway engineering, metagenomic screening, microbial genomes, extremophiles, rational design, directed evolution, and more Provides a holistic approach to the research of microbial enzymes

Solid-state Fermentation

Solid-state Fermentation
  • Author : Ashok Pandey
  • Publisher :Unknown
  • Release Date :1994
  • Total pages :188
  • ISBN : 8122406610
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Summary : Papers presented at Specialist Group Meeting & Symposium on Solid State Fermentation, held at Trivandrum, during March 23-24, 1994, organized by the Regional Research Laboratory, Trivandrum.

Application of Neural Networks to Fed-batch Fermentation

Application of Neural Networks to Fed-batch Fermentation
  • Author : Tammy Yut-Ling Chan
  • Publisher :Unknown
  • Release Date :2005
  • Total pages :476
  • ISBN : OCLC:60316332
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Summary :

Advances in Bioprocess Engineering

Advances in Bioprocess Engineering
  • Author : Enrique Galindo,Octavio R. Ramírez
  • Publisher :Unknown
  • Release Date :2013-04-17
  • Total pages :275
  • ISBN : 9789401706438
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Summary : Bioprocess engineering has played a key role in biotechnology, contributing towards bringing the exciting new discoveries of molecular and cellular biology into the applied sphere, and in maintaining established processes, some centuries-old, efficient and essential for today's industry. Novel developments and new application areas of biotechnology, along with increasing constraints in costs, product quality, regulatory and environmental considerations, have placed the biochemical engineer at the forefront of new challenges. This second volume of Advances in Bioprocess Engineering reflects precisely the multidisciplinary nature of the field, where new and traditional areas of application are nurtured by a better understanding of fundamental phenomena and by the utilization of novel techniques and methodologies. The chapters in this book were written by the invited speakers to the 2nd International Symposium on Bioprocess Engineering, Mazatlan, Mexico, September 1997.

Gene Expression in Recombinant Microorganisms

Gene Expression in Recombinant Microorganisms
  • Author : Alan Smith
  • Publisher :Unknown
  • Release Date :1994-11-23
  • Total pages :432
  • ISBN : 0824795431
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Summary : Describing the scientific and commercial applications of microbial recombinant DNA technology, this outstanding, single-source reference offers state-of-the-art reviews of gene expression in the most important classes of recombinant microorganisms-providing numerous examples of the expression of homologous genes or heterologous gene products. Presents a unique collection of safety and regulatory considerations from around the world and addresses specific measures to be taken for large-scale industrial operations!

The Systematic Consideration of the Large-scale Fed-batch Fermentation Inhomogeneities Using a Genetically Modified C. Glutamicum Strain as a Model Organism

The Systematic Consideration of the Large-scale Fed-batch Fermentation Inhomogeneities Using a Genetically Modified C. Glutamicum Strain as a Model Organism
  • Author : Williams C. Olughu
  • Publisher :Unknown
  • Release Date :2018
  • Total pages :229
  • ISBN : OCLC:1085191171
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Summary :