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Laboratory Methods in Microfluidics

Laboratory Methods in Microfluidics
  • Author : Basant Giri
  • Publisher :Unknown
  • Release Date :2017-05-15
  • Total pages :178
  • ISBN : 9780128132364
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Summary : Laboratory Methods in Microfluidics features a range of lab methods and techniques necessary to fully understand microfluidic technology applications. Microfluidics deals with the manipulation of small volumes of fluids at sub-millimeter scale domain channels. This exciting new field is becoming an increasingly popular subject both for research and education in various disciplines of science, including chemistry, chemical engineering and environmental science. The unique properties of microfluidic technologies, such as rapid sample processing and precise control of fluids in assay have made them attractive candidates to replace traditional experimental approaches. Practical for students, instructors, and researchers, this book provides a much-needed, comprehensive new laboratory reference in this rapidly growing and exciting new field of research. Provides a number of detailed methods and instructions for experiments in microfluidics Features an appendix that highlights several standard laboratory techniques, including reagent preparation plus a list of materials vendors for quick reference Authored by a microfluidics expert with nearly a decade of research on the subject

Microfluidics in Detection Science

Microfluidics in Detection Science
  • Author : Fatima H Labeed,Henry O Fatoyinbo
  • Publisher :Unknown
  • Release Date :2014-10-24
  • Total pages :281
  • ISBN : 9781849737609
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Summary : The concept of a miniaturised laboratory on a disposable chip is now a reality, and in everyday use in industry, medicine and defence. New devices are launched all the time, prompting the need for a straightforward guide to the design and manufacture of lab-on-a-chip (LOC) devices. This book presents a modular approach to the construction and integration of LOC components in detection science. The editors have brought together some of the leading experts from academia and industry to present an accessible guide to the technology available and its potential. Several chapters are devoted to applications, presenting both the sampling regime and detection methods needed. Further chapters describe the integration of LOC devices, not only with each other but also into existing technologies. With insights into LOC applications, from biosensing to molecular and chemical analysis, and presenting scaled-down versions of existing technology alongside unique approaches that exploit the physics of the micro and nano-scale, this book will appeal to newcomers to the field and practitioners requiring a convenient reference.

Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery

Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery
  • Author : Paul C.H. Li
  • Publisher :Unknown
  • Release Date :2005-11-01
  • Total pages :528
  • ISBN : 9781420027457
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Summary : The microfluidic lab-on-a-chip allows scientists to conduct chemical and biochemical analysis in a miniaturized format so small that properties and effects are successfully enhanced, and processes seamlessly integrated. This microscale advantage translates into greater sensitivity, more accurate results, and better information. Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery focuses on all aspects of the microfluidic lab-on-a-chip technologies and offers an overview of the available technology, its limitations, and its breakthroughs over the years. It emphasizes analytical applications of microfluidic technology and offers in-depth coverage of micromachining methods, microfluidic operations, chemical separations, sample preparation and injection methods, detection technology, and various chemical and biological analyses. Other topics of interest include the use of polymeric chips, fluid flow valve and control, single-cell analysis, DNA and RNA amplification techniques, DNA hybridization, immunoassays and enzymatic assays. Originally conceived as a single chapter published in Ewing's Analytical Instrumentation, this book is a gateway to the vast literature and conference proceedings on the topic. Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery expands upon its roots to present a comprehensive treatment of microfluidic lab-on-a-chip methods and applications for novices and advanced researchers alike.

Lab-on-a-chip

Lab-on-a-chip
  • Author : Albert Berg
  • Publisher :Unknown
  • Release Date :2003
  • Total pages :394
  • ISBN : 9780444511003
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Summary : In the past ten years there has been a rapid growth of the research and application area known as Lab-on-a-Chip. After an initial focus on electrokinetic separation techniques on chip, the scope of the field has widened to include topics like microfluidics, DNA analysis, cell analysis, microreactors and mass spectrometer interfacing. As well as the analytical chemistry community, synthetic chemists, chemical engineers, biochemists and biomedical engineers are now also becoming more and more interested in using new micro- and nanotechnological techniques. This first Lab-on-a-Chip book contains a broad collection of papers on microtechnology, microfluidics, analytical methods and applications. All contributions are written by leading researchers in their respective fields, and provide new scientists with an overview of the field, to make him/her aware of the enormous opportunities offered by modern technology. The work presented in this book will definitely stimulate readers to new ideas and concepts, and lead to further innovations in this area. - Provides a quick introduction into the different aspects of this field - Describes technology that has already revolutionized the world of chemical and biochemical analysis and synthesis - All contributions are written by leading researchers in their respective fields

Nanofluidics and Microfluidics

Nanofluidics and Microfluidics
  • Author : Shaurya Prakash,Junghoon Yeom
  • Publisher :Unknown
  • Release Date :2014-01-16
  • Total pages :312
  • ISBN : 9781437744705
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Summary : To provide an interdisciplinary readership with the necessary toolkit to work with micro- and nanofluidics, this book provides basic theory, fundamentals of microfabrication, advanced fabrication methods, device characterization methods and detailed examples of applications of nanofluidics devices and systems. Case studies describing fabrication of complex micro- and nanoscale systems help the reader gain a practical understanding of developing and fabricating such systems. The resulting work covers the fundamentals, processes and applied challenges of functional engineered nanofluidic systems for a variety of different applications, including discussions of lab-on-chip, bio-related applications and emerging technologies for energy and environmental engineering. The fundamentals of micro- and nanofluidic systems and micro- and nanofabrication techniques provide readers from a variety of academic backgrounds with the understanding required to develop new systems and applications. Case studies introduce and illustrate state-of-the-art applications across areas, including lab-on-chip, energy and bio-based applications. Prakash and Yeom provide readers with an essential toolkit to take micro- and nanofluidic applications out of the research lab and into commercial and laboratory applications.

Lab-on-a-Chip Devices and Micro-Total Analysis Systems

Lab-on-a-Chip Devices and Micro-Total Analysis Systems
  • Author : Jaime Castillo-León,Winnie E. Svendsen
  • Publisher :Unknown
  • Release Date :2014-11-05
  • Total pages :243
  • ISBN : 9783319086873
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Summary : This book covers all the steps in order to fabricate a lab-on-a-chip device starting from the idea, the design, simulation, fabrication and final evaluation. Additionally, it includes basic theory on microfluidics essential to understand how fluids behave at such reduced scale. Examples of successful histories of lab-on-a-chip systems that made an impact in fields like biomedicine and life sciences are also provided. This book also: · Provides readers with a unique approach and toolset for lab-on-a-chip development in terms of materials, fabrication techniques, and components · Discusses novel materials and techniques, such as paper-based devices and synthesis of chemical compounds on-chip · Covers the four key aspects of development: basic theory, design, fabrication, and testing · Provides readers with a comprehensive list of the most important journals, blogs, forums, and conferences where microfluidics and lab-on-a-chip news, methods, techniques and challenges are presented and discussed, as well as a list of companies providing design and simulation support, components, and/or developing lab-on-a-chip and microfluidic devices.

Microfluidics for Pharmaceutical Applications

Microfluidics for Pharmaceutical Applications
  • Author : Helder A. Santos,Dongfei Liu,Hongbo Zhang
  • Publisher :Unknown
  • Release Date :2018-10-12
  • Total pages :495
  • ISBN : 9780128126608
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Summary : Microfluidics for Pharmaceutical Applications: From Nano/Micro Systems Fabrication to Controlled Drug Delivery is a concept-orientated reference that features case studies on utilizing microfluidics for drug delivery applications. It is a valuable learning reference on microfluidics for drug delivery applications and assists practitioners developing novel drug delivery platforms using microfluidics. It explores advances in microfluidics for drug delivery applications from different perspectives, covering device fabrication, fluid dynamics, cutting-edge microfluidic technology in the global drug delivery industry, lab-on-chip nano/micro fabrication and drug encapsulation, cell encapsulation and delivery, and cell- drug interaction screening. These microfluidic platforms have revolutionized the drug delivery field, but also show great potential for industrial applications. Presents detailed coverage on the fabrication of novel drug delivery systems with desired characteristics, such as uniform size, Janus particles, and particular or combined responsiveness Includes a variety of case studies that explain principles Focuses on commercialization, cost, safety, society and educational issues of microfluidic applications, showing how microfluidics is used in the real world

Microfluidics and Lab-on-a-Chip

Microfluidics and Lab-on-a-Chip
  • Author : Andreas Manz,Giuseppina Simone,Jonathan S. O'Connor,Pavel Neuzil
  • Publisher :Unknown
  • Release Date :2020-09-24
  • Total pages :280
  • ISBN : 9781782628330
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Summary : Responding to the need for an affordable, easy-to-read textbook that introduces microfluidics to undergraduate and postgraduate students, this concise book will provide a broad overview of the important theoretical and practical aspects of microfluidics and lab-on-a-chip, as well as its applications.

Microfluidic Diagnostics

Microfluidic Diagnostics
  • Author : Gareth Jenkins,Colin D. Mansfield
  • Publisher :Unknown
  • Release Date :2013-01-18
  • Total pages :525
  • ISBN : 1627031332
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Summary : Microfluidic techniques are becoming widely incorporated into medical diagnostic systems due to the inherent advantages of miniaturization. In Microfluidic Diagnostics: Methods in Molecular Biology, researchers in the field detail methods and protocols covering subjects such as microfluidic device fabrication, on-chip sample preparation, diagnostic applications and detection methodologies. The protocols described range from cutting-edge developments to established techniques and basic demonstrations suitable for education and training; from basic fabrication methods to commercializing research. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microfluidic Diagnostics: Methods in Molecular Biology seeks to aid scientists in the further development and commercialization of microfluidic diagnostic technologies

Fundamentals of Microfluidics and Lab on a Chip for Biological Analysis and Discovery

Fundamentals of Microfluidics and Lab on a Chip for Biological Analysis and Discovery
  • Author : Paul C.H. Li
  • Publisher :Unknown
  • Release Date :2010-02-24
  • Total pages :418
  • ISBN : 9781439882177
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Summary : Lab-on-a-chip technology permits us to make many important discoveries that can only be observed at the microscale or the nanoscale. Using this technology, biological and biochemical analyses translate into greater sensitivity, more accurate results, and more valuable findings. Authored by one of the field’s pioneering researchers, Fundamentals of Microfluidics and Lab on a Chip for Biological Analysis and Discovery focuses on all key aspects of microfluidic lab-on-a-chip technologies to offer an exceptionally cohesive overview of the science, its limitations, breakthroughs made over the years, and currently emerging advances. The book emphasizes analytical applications of microfluidic technology and offers in-depth coverage of micromachining methods, microfluidic operations, chemical separations, sample preparation and injection methods, detection technology, and various chemical and biological analyses. Other topics of interest include the use of polymeric chips, fluid flow valve and control, single-cell analysis, DNA and RNA amplification techniques, DNA hybridization, immunoassays, and enzymatic assays. The book includes more than 300 figures that depict novel chip functions and breakthroughs and 16 tables summarize materials and refer readers to additional resources. An appendix compiles extensive analytical applications from emerging and established research groups. Beginners in the field will find the book useful for navigating the vast literature related to the technology, while experienced researchers will rely on the compiled information for easy comparison and references for further study. Derived from the highly popular Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery (2006), this volume is also readily adaptable for classroom use. Problem sets in each chapter help students test their assimilation of the material and clarify challenging concepts. The book contains a comprehensive glossary, a complete index, and extensive references. A solutions manual is available with qualifying course adoption.

Modeling and Measurement Methods for Acoustic Waves and for Acoustic Microdevices

Modeling and Measurement Methods for Acoustic Waves and for Acoustic Microdevices
  • Author : Marco G. Beghi
  • Publisher :Unknown
  • Release Date :2013-08-28
  • Total pages :614
  • ISBN : 9789535111894
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Summary : Acoustics is a mature field which enjoys a never ending youth. New developments are induced by either the search for a better understanding, or by technological innovations. Micro-fabrication techniques introduced a whole new class of microdevices, which exploit acoustic waves for various tasks, and in particular for information processing and for sensing purposes. Performance improvements are achievable by better modelling tools, able to deal with more complex configurations, and by more refined techniques of fabrication and of integration in technological systems, like wireless communications. Several chapters of this book deal with modelling and fabrication techniques for microdevices, including unconventional phenomena and configurations. But this is far from exhausting the research lines in acoustics. Theoretical analyses and modelling techniques are presented, for phenomena ranging from the detection of cracks to the acoustics of the oceans. Measurement methods are also discussed, which probe by acoustic waves the properties of widely different systems.

Electrokinetics in Microfluidics

Electrokinetics in Microfluidics
  • Author : Dongqing Li
  • Publisher :Unknown
  • Release Date :2004-08-20
  • Total pages :652
  • ISBN : 0080530745
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Summary : A lab-on-a-chip device is a microscale laboratory on a credit-card sized glass or plastic chip with a network of microchannels, electrodes, sensors and electronic circuits. These labs on a chip can duplicate the specialized functions as performed by their room-sized counterparts, such as clinical diagnoses, PCR and electrophoretic separation. The advantages of these labs on a chip include significant reduction in the amounts of samples and reagents, very short reaction and analysis time, high throughput and portability. Generally, a lab-on-a-chip device must perform a number of microfluidic functions: pumping, mixing, thermal cycling/incubating, dispensing, and separating. Precise manipulation of these microfluidic processes is key to the operation and performance of labs on a chip. The objective of this book is to provide a fundamental understanding of the interfacial electrokinetic phenomena in several key microfluidic processes, and to show how these phenomena can be utilised to control the microfluidic processes. For this purpose, this book emphasises the theoretical modelling and the numerical simulation of these electrokinetic phenomena in microfluidics. However, experimental studies of the electrokinetic microfluidic processes are also highlighted in sufficient detail. The first book which systematically reviews electrokinetic microfluidics processes for lab-on-a chip applications Covers modelling and numerical simulation of the electrokinetic microfluidics processes Providing information on experimental studies and details of experimental techniques, which are essential for those who are new to this field

Laboratory Methods in Dynamic Electroanalysis

Laboratory Methods in Dynamic Electroanalysis
  • Author : M. Teresa Fernández Abedul
  • Publisher :Unknown
  • Release Date :2019-10-13
  • Total pages :408
  • ISBN : 9780128159330
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Summary : Laboratory Methods in Dynamic Electroanalysis is a useful guide to introduce analytical chemists and scientists of related disciplines to the world of dynamic electroanalysis using simple and low-cost methods. The trend toward decentralization of analysis has made this fascinating field one of the fastest-growing branches of analytical chemistry. As electroanalytical devices have moved from conventional electrochemical cells (10-20 mL) to current cells (e.g. 5-50 mL) based on different materials such as paper or polymers that integrate thick- or thin-film electrodes, interesting strategies have emerged, such as the combination of microfluidic cells and biosensing or nanostructuration of electrodes. This book provides detailed, easy procedures for dynamic electroanalysis and covers the main trends in electrochemical cells and electrodes, including microfluidic electrodes, electrochemical detection in microchip electrophoresis, nanostructuration of electrodes, development of bio (enzymatic, immuno, and DNA) assays, paper-based electrodes, interdigitated array electrodes, multiplexed analysis, and combination with optics. Different strategies and techniques (amperometric, voltammetric, and impedimetric) are presented in a didactic, practice-based way, and a bibliography provides readers with additional sources of information. Provides easy-to-implement experiments using low-cost, simple equipment Includes laboratory methodologies that utilize both conventional designs and the latest trends in dynamic electroanalysis Goes beyond the fundamentals covered in other books, focusing instead on practical applications of electroanalysis

Introduction to Microfluidics

Introduction to Microfluidics
  • Author : Patrick Tabeling
  • Publisher :Unknown
  • Release Date :2010-05-06
  • Total pages :312
  • ISBN : 9780199588169
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Summary : Microfluidics is a young discipline which enables scientists and engineers to handle fluids in the biochips of the future.The book is an introduction to this discipline. It presents in simple terms the most important notions of the domain: how fluids move on the chip, conveying materials, molecules, electrical charges, and heat.

Microfluidic Techniques

Microfluidic Techniques
  • Author : Shelley D. Minteer
  • Publisher :Unknown
  • Release Date :2006
  • Total pages :247
  • ISBN : 9781592599974
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Summary : This volume highlights recent important advances in microfluidic techniques for biological applications. Opening chapters focus on the most popular techniques for fabrication of microchips (photolithography, laser ablation, and soft lithography), while further sections focus on microfluidic techniques for bioanalytical assays and bioprocesses, such as DNA analysis, PCR, immunoassays, and cell reactors. Microfluidic Techniques will provide molecular biologists and biochemists with the state-of-the-art technical information required to perform microscale bioassays and bioprocessing in the laboratory.

Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis

Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2018-11-22
  • Total pages :264
  • ISBN : 9780128142851
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Summary : Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Laboratory Methods in Dynamic Electroanalysis

Laboratory Methods in Dynamic Electroanalysis
  • Author : M. Teresa Fernández Abedul
  • Publisher :Unknown
  • Release Date :2019-10-13
  • Total pages :408
  • ISBN : 9780128159330
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Summary : Laboratory Methods in Dynamic Electroanalysis is a useful guide to introduce analytical chemists and scientists of related disciplines to the world of dynamic electroanalysis using simple and low-cost methods. The trend toward decentralization of analysis has made this fascinating field one of the fastest-growing branches of analytical chemistry. As electroanalytical devices have moved from conventional electrochemical cells (10-20 mL) to current cells (e.g. 5-50 mL) based on different materials such as paper or polymers that integrate thick- or thin-film electrodes, interesting strategies have emerged, such as the combination of microfluidic cells and biosensing or nanostructuration of electrodes. This book provides detailed, easy procedures for dynamic electroanalysis and covers the main trends in electrochemical cells and electrodes, including microfluidic electrodes, electrochemical detection in microchip electrophoresis, nanostructuration of electrodes, development of bio (enzymatic, immuno, and DNA) assays, paper-based electrodes, interdigitated array electrodes, multiplexed analysis, and combination with optics. Different strategies and techniques (amperometric, voltammetric, and impedimetric) are presented in a didactic, practice-based way, and a bibliography provides readers with additional sources of information. Provides easy-to-implement experiments using low-cost, simple equipment Includes laboratory methodologies that utilize both conventional designs and the latest trends in dynamic electroanalysis Goes beyond the fundamentals covered in other books, focusing instead on practical applications of electroanalysis

Microfluidics for Biologists

Microfluidics for Biologists
  • Author : Chandra K. Dixit,Ajeet Kaushik
  • Publisher :Unknown
  • Release Date :2016-10-13
  • Total pages :252
  • ISBN : 9783319400365
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Summary : This book describes novel microtechnologies and integration strategies for developing a new class of assay systems to retrieve desired health information from patients in real-time. The selection and integration of sensor components and operational parameters for developing point-of-care (POC) are also described in detail. The basics that govern the microfluidic regimen and the techniques and methods currently employed for fabricating microfluidic systems and integrating biosensors are thoroughly covered. This book also describes the application of microfluidics in the field of cell and molecular biology, single cell biology, disease diagnostics, as well as the commercially available systems that have been either introduced or have the potential of being used in research and development. This is an ideal book for aiding biologists in understanding the fundamentals and applications of microfluidics. This book also: Describes the preparatory methods for developing 3-dimensional microfluidic structures and their use for Lab-on-a-Chip design Explains the significance of miniaturization and integration of sensing components to develop wearable sensors for point-of-care (POC) Demonstrates the application of microfluidics to life sciences and analytical chemistry, including disease diagnostics and separations Motivates new ideas related to novel platforms, valving technology, miniaturized transduction methods, and device integration to develop next generation sequencing Discusses future prospects and challenges of the field of microfluidics in the areas of life sciences in general and diagnostics in particular

Microfluidic Cell Culture Systems

Microfluidic Cell Culture Systems
  • Author : Christopher Bettinger,Jeffrey T Borenstein,Sarah L Tao
  • Publisher :Unknown
  • Release Date :2012-12-31
  • Total pages :452
  • ISBN : 9781437734607
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Summary : The fields of microfluidics and BioMEMS are significantly impacting cell biology research and applications through the application of engineering solutions to human disease and health problems. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology. This new professional reference applies the techniques of microsystems to cell culture applications. The authors provide a thoroughly practical guide to the principles of microfluidic device design and operation and their application to cell culture techniques. The resulting book is crammed with strategies and techniques that can be immediately deployed in the lab. Equally, the insights into cell culture applications will provide those involved in traditional microfluidics and BioMEMS with an understanding of the specific demands and opportunities presented by biological applications. The goal is to guide new and interested researchers and technology developers to the important areas and state-of-the-practice strategies that will enhance the efficiency and value of their technologies, devices and biomedical products. Provides insights into the design and development of microfluidic systems with a specific focus on cell culture applications Focuses on strategies and techniques for the design and fabrication of microfluidic systems and devices for cell culture Provides balanced coverage of microsystems engineering and bioengineering

Microfluidic Devices for Biomedical Applications

Microfluidic Devices for Biomedical Applications
  • Author : Xiujun James Li,Yu Zhou
  • Publisher :Unknown
  • Release Date :2013-10-31
  • Total pages :676
  • ISBN : 9780857097040
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Summary : Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic devices for biomedical applications discusses the fundamentals of microfluidics and explores in detail a wide range of medical applications. The first part of the book reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focussing on the materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles. Part three considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The final part of the book covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis. Microfluidic devices for biomedical applications is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries. Discusses the fundamentals of microfluidics or lab-on-a-chip (LOC) and explores in detail a wide range of medical applications Considers materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies Considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering

Design Automation Methods and Tools for Microfluidics-Based Biochips

Design Automation Methods and Tools for Microfluidics-Based Biochips
  • Author : Jun Zeng
  • Publisher :Unknown
  • Release Date :2006-11-08
  • Total pages :403
  • ISBN : 9781402051234
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Summary : Design Automation Methods and Tools for Microfluidics-Based Biochips deals with all aspects of design automation for microfluidics-based biochips. Experts have contributed chapters on many aspects of biochip design automation. Topics covered include: device modeling; adaptation of bioassays for on-chip implementations; numerical methods and simulation tools; architectural synthesis, scheduling and binding of assay operations; physical design and module placement; fault modeling and testing; and reconfiguration methods.