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Introduction to Modern Liquid Chromatography

Introduction to Modern Liquid Chromatography
  • Author : Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan
  • Publisher :Unknown
  • Release Date :2011-09-20
  • Total pages :960
  • ISBN : 1118210395
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Summary : The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

High-Performance Liquid Chromatography in Endocrinology

High-Performance Liquid Chromatography in Endocrinology
  • Author : H.L.J. Makin,R. Newton
  • Publisher :Unknown
  • Release Date :2012-12-06
  • Total pages :262
  • ISBN : 3642834671
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Summary : This book originates from a symposium held at the London Hospital Medical College under the auspices of Applied Chromatography Systems Ltd. to discuss the place of HPLC in the endocrinology field. Many of the authors of the present book were speakers at this symposium. It seemed to us that many endocrinolo gists did not at that time fully appreciate the value of HPLC, and this book was designed to publicise the potential value of this technique. A survey of methods used in the steroid field Cp 185) confirmed the view that HPLC is not being used as widely, particularly in research described in clinical journals, as might be expected. We hope that this book will illustrate, albeit in a few selected areas of endocrinology, just how versatile and powerful a technique HPLC is, and en courage those who have not yet experienced it to have a go. The beginner does not need to buy expensive instrumentation - all that is required is a pump, injector, column and detector - the rest can come later! All the authors have practical experience in the use of HPLC in the particular area they discuss. All readers who discover apparent errors or who feel that the treatment of a topic of interest can be improved upon are encouraged to contact the editors. All criti cism, especially if constructive, is welcomed. We are still learning, and other peoples' experience is always valuable.

High Performance Liquid Chromatography

High Performance Liquid Chromatography
  • Author : Win Fung Ho,Brian Stuart,Elizabeth R. Prichard
  • Publisher :Unknown
  • Release Date :2003
  • Total pages :35
  • ISBN : 9780854044832
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Summary : For those new to this technique, this guide provides basic tips, key skills, awareness and guidance on good practice of High Performance Liquid Chromatography (HPLC). It will help build understanding of the important issues to consider during analysis and how to develop further skills. HPLC is one of the most widely used techniques in industry to separate and analyse compounds through the mass transfer of analytes between stationary and mobile phases. This guide will prove an invaluable introduction to the technique. Starting with a look at the basic theory, the guide goes on to describe HPLC components, system parameters, suitability checks and testing. Later sections cover calibration, problem solving and data handling. High Performance Liquid Chromatography is one of the Practical Laboratory Skills Training Guides, a series that aims to make achieving best practice easy. These invaluable manuals will enable both experienced and inexperienced staff to get the essential basics of any experiment right simply by following the clear and easy to use instructions provided. The guides are written by experienced scientists and include minimal theory, plenty of practical exercises in order to assess competence, and trouble shooting information. Other titles are: Measurement of Mass; Measurement of Volume; Measurement of pH; and Gas Chromatography.

Applications of High Performance Liquid Chromatography

Applications of High Performance Liquid Chromatography
  • Author : S. Pryde,M.T. Gilbert
  • Publisher :Unknown
  • Release Date :1979-01-31
  • Total pages :256
  • ISBN : 9780412142208
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Summary :

Liquid Chromatography

Liquid Chromatography
  • Author : Salvatore Fanali,Paul R. Haddad,Colin Poole,Marja-Liisa Riekkola
  • Publisher :Unknown
  • Release Date :2017-06-22
  • Total pages :808
  • ISBN : 0128093455
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Summary : Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

Protein Liquid Chromatography

Protein Liquid Chromatography
  • Author : M. Kastner
  • Publisher :Unknown
  • Release Date :2000
  • Total pages :941
  • ISBN : 9780444502117
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Summary : Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Liquid Chromatography - Mass Spectrometry

Liquid Chromatography - Mass Spectrometry
  • Author : Robert E. Ardrey
  • Publisher :Unknown
  • Release Date :2003-04-02
  • Total pages :300
  • ISBN : 9780471498018
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Summary : First explaining the basic principles of liquid chromatography and mass spectrometry and then discussing the current applications and practical benefits of LC-MS, along with descriptions of the basic instrumentation, this title will prove to be the indispensable reference source for everyone wishing to use this increasingly important tandem technique. * First book to concentrate on principles of LC-MS * Explains principles of mass spectrometry and chromatography before moving on to LC-MS * Describes instrumental aspects of LC-MS * Discusses current applications of LC-MS and shows benefits of using this technique in practice

Liquid Chromatography of Natural Pigments and Synthetic Dyes

Liquid Chromatography of Natural Pigments and Synthetic Dyes
  • Author : Tibor Cserháti
  • Publisher :Unknown
  • Release Date :2006-12-07
  • Total pages :602
  • ISBN : 9780080465760
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Summary : This latest volume in the series entitled Liquid Chromatography of Natural Pigments and Synthetic Dyes presents an overview of the latest developments in the field while critically evaluating this method of analysis and providing comparisons of the various liquid chromatographic separation techniques that are currently available. Natural pigments and synthetic dyes are extensively used in various fields of everyday life including food production, textile industry, paper production, agricultural practice and research and water science and technology. Besides their capacity for increasing the marketability of products, natural pigments have shown advantageous biological activity as antioxidants and anticancer agents. On the negative side, synthetic pigments have a significant impact on the environment and can cause adverse toxicological side effects. Both pigment classes exhibit considerable structural diversity. As the stability of the pigments against hydrolysis, oxidation and other environmental and technological conditions is markedly different, the exact determination of the pigment composition may help for the prediction of the shelf-life of products and the assessment of the influence of technological steps on the pigment fractions resulting in more consumer friend processing methods. Furthermore, the qualitative determination and identification of the pigments may contribute to the establishment of the provenance of the product. The unique separation capacity of liquid chromatographic (LC) techniques makes it a method of preference for the analysis of pigments in any complicated accompanying matrices. * an overview of the latest developments in the field * a critical evaluation of results from this form of analysis * a comparison of the various LC (liquid chromatographic) separation techniques * future trends in the LC analysis of pigments

Liquid Chromatography/Mass Spectrometry

Liquid Chromatography/Mass Spectrometry
  • Author : Alfred L. Yergey,Charles G. Edmonds,Ivor A.S. Lewis,Marvin L. Vestal
  • Publisher :Unknown
  • Release Date :1990-01-31
  • Total pages :306
  • ISBN : 9780306431869
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Summary : This book is intended both to be an introduction to techniques and applications of liquid chromatography/mass spectrometry and to serve as a reference for future workers. When we undertook its writing, we chose not to cover the field, particularly applications, exhaustively. Rather we wished to produce a book that would be of use to people just beginning to use the technique as well as to more advanced practitioners. In this regard, we have sought to highlight techniques and applications that are of current importance, while not neglecting descriptions of approaches that may be of significance in the future. We hope that we have succeeded in this. At the same time we hope that the bibliography, with indexes classified by author and title, will make this book of value to those who may disagree with our emphasis. ACKNOWLEDGMENTS. One of us (C. G. E. ) wishes to acknowledge the en couragment of Professor J. A. McCloskey in undertaking this project. All four of us are grateful for the continuous and expert assistance of V. A. Edmonds in the preparation of the Bibliography. Alfred L. Y ergey Bethesda, Maryland Charles G. Edmonds Richland, Washington lvor A. S. Lewis London, England Marvin L. Vestal Houston, Texas v Contents 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . 1 . . . . . . . . . . . 2. Direct Liquid Introduction Interfaces . . . . . . . . . . 5 . . . 2. 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2. 2. Operating Principles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2. 3. Specific DLI Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3. 1. Capillary Inlets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3. 2. Diaphragm Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2. 3. 3. Nebulizing Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Liquid Chromatography Detectors

Liquid Chromatography Detectors
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2011-09-22
  • Total pages :247
  • ISBN : 9780080858111
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Summary : Liquid Chromatography Detectors

Modern Liquid Chromatography of Macromolecules

Modern Liquid Chromatography of Macromolecules
  • Author : B.G. Belenkii,L.Z. Vilenchik
  • Publisher :Unknown
  • Release Date :2000-04-01
  • Total pages :431
  • ISBN : 9780080858289
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Summary : Modern Liquid Chromatography of Macromolecules

Instrumental Liquid Chromatography

Instrumental Liquid Chromatography
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2011-09-22
  • Total pages :328
  • ISBN : 9780080858050
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Summary : Instrumental Liquid Chromatography

High Performance Liquid Chromatography

High Performance Liquid Chromatography
  • Author : W.J. Lough,I.W. Wainer
  • Publisher :Unknown
  • Release Date :1995-09-30
  • Total pages :288
  • ISBN : 9780751400762
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Summary : High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Polymer Characterization by Liquid Chromatography

Polymer Characterization by Liquid Chromatography
  • Author : G. Glöckner
  • Publisher :Unknown
  • Release Date :1987-02-01
  • Total pages :585
  • ISBN : 9780080858371
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Summary : The main subject of this book is the characterization of plastics. To a high degree the properties of these polymers depend on the distribution of the molar mass and of other structural features, and small deviations frequently have a great effect. Therefore the characterization of polymers cannot be restricted to the determination of mean values but must yield information on these distributions. Using classical methods, the analytical fractionation of polymer homologues and structurally isomeric polymers is extremely time-consuming. Therefore, efficient chromatographic techniques are being increasingly employed in modern polymer characterization. In the first place, high-performance liquid chromatography is applied, in the form of size exclusion chromatography. It is also possible, however, to use other separation modes. More space is devoted to these other possibilities in this volume than is merited by their current range of applications, as the author believes that many separation problems will be solved by separation techniques of the non-exclusion type. Nevertheless, much emphasis is placed on size exclusion chromatography. Not only because of its current wide range of applications, but also because its relative importance, as a complement to other chromatographic techniques may even increase in the forthcoming years. This book is the first to cover all phenomena related to the above considerations. Starting with an introduction to basic liquid chromatography and to polymer science, it deals with the adsorption behaviour of polymers, with gradient techniques, with the kinetic band broadening in liquid chromatography, with instrumental features and packing materials. The book consists of four balanced sections and related information from about 1800 references is compiled in the tables. Some 250 figures and 30 tables will help give the reader a clear insight of the topics discussed. The book is aimed at helping the analyst or polymer chemist who is looking for information about chromatographic methods for the characterization of polymers.

Micellar Liquid Chromatography

Micellar Liquid Chromatography
  • Author : Alain Berthod,Celia Garcia-Alvarez-Coque
  • Publisher :Unknown
  • Release Date :2000-03-30
  • Total pages :632
  • ISBN : 9780824799939
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Summary : Micellar Liquid Chromatography reviews the use of surfactant solutions at or above the critical micelle concentration as mobile phases in liquid chromatography. It employs a computer-assisted optimization methodology and integrates micellar liquid chromatography (MLC) with other chromatographic and electrophoretic techniques using surfactants. It also includes the MICHROM software package on CD-ROM to facilitate the application of equations and optimize efficiency of MLC systems.

High Performance Liquid Chromatography in Phytochemical Analysis

High Performance Liquid Chromatography in Phytochemical Analysis
  • Author : Monika Waksmundzka-Hajnos,Joseph Sherma
  • Publisher :Unknown
  • Release Date :2010-11-08
  • Total pages :996
  • ISBN : 9781420092615
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Summary : The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a comp

High-performance Liquid Chromatography: Optimization in liquid chromatography

High-performance Liquid Chromatography: Optimization in liquid chromatography
  • Author : Csaba Horváth
  • Publisher :Unknown
  • Release Date :1980
  • Total pages :341
  • ISBN : 09876543XX
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Summary : Optimization in liquid chromatography. Liquid chromatography on silica and alumina as stationary phases. Reserved-phase chromatography.

High Performance Liquid Chromatography

High Performance Liquid Chromatography
  • Author : Sandie Lindsay,Barnes,John Barnes,ACOL (Project)
  • Publisher :Unknown
  • Release Date :1992-07-27
  • Total pages :337
  • ISBN : 9780471931157
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Summary : Part of a series of textbooks concerned with the training, continuing education and updating of all technical staff concerned with analytical chemistry, this volume explores a powerful chromatographic technique which has arisen from research carried out on liquid and gas chromatography.

Liquid Chromatography-Mass Spectrometry

Liquid Chromatography-Mass Spectrometry
  • Author : Wilfried M.A. Niessen
  • Publisher :Unknown
  • Release Date :2006-08-09
  • Total pages :632
  • ISBN : 9781420014549
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Summary : A constructive evaluation of the most significant developments in liquid chromatography-mass spectrometry (LC-MS) and its uses for quantitative bioanalysis and characterization for a diverse range of disciplines, Liquid Chromatography-Mass Spectrometry, Third Edition offers a well-rounded coverage of the latest technological developments and

Liquid Chromatography Detectors

Liquid Chromatography Detectors
  • Author : T. M. Vickrey
  • Publisher :Unknown
  • Release Date :1983-07-21
  • Total pages :448
  • ISBN : 9780824719166
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Summary : This book documents the principles of operation, the advantages and drawbacks, and the potential future of currently available liquid chromatographic detectors. In offering a snapshot of the current technology, it provides clear explanations and possible new horizons for both beginners and experts.

Liquid Chromatography for the Analyst

Liquid Chromatography for the Analyst
  • Author : Raymond P.W. Scott
  • Publisher :Unknown
  • Release Date :1994-01-17
  • Total pages :344
  • ISBN : 9780824791841
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Summary : This work provides a clear presentation of the chromatographic process - demonstrating the functions of all associated instrumentation and the procedures necessary to obtain accurate qualitative and quantitative results. The work supplies a host of applications from a variety of sources to help identify the best equipment, the most appropriate columns and the most suitable phase systems for specific samples.