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Mammalian Transcription Analysis and Methods

Mammalian Transcription Analysis and Methods
  • Author : Ananda L. Roy
  • Publisher :Unknown
  • Release Date :2020-04-01
  • Total pages :291
  • ISBN : 0080931138
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Summary : This volume emphasizes analysis and methods for transcription studies. Analysis is covered at both the tissue and molecular level, with a special section on tissue specific transription factors. Over 15 methods are covered, including immunohistochemistry, fluorescence, genomic analysis methodlogy, chromatin remodeling, as well as genetic array/chip analysis.

Transcriptional Regulation

Transcriptional Regulation
  • Author : Ales Vancura
  • Publisher :Unknown
  • Release Date :2011-11-24
  • Total pages :627
  • ISBN : 1617793752
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Summary : Through many recent remarkable developments, perhaps the most significant advancements in the study of transcriptional regulation are the development of genome-wide approaches for measuring gene expression, exemplified by gene chips (chip), and chromatin immunoprecipitation assays (ChIP) for measuring in vivo protein-DNA interactions at any genomic loci. Transcriptional Regulation: Methods and Protocols takes this progress and builds upon it with a collection of key protocols used in expert laboratories around the world. Divided into four convenient sections, this detailed volume explores promoter elements, transcription factors, and preinitiation complex (PIC) assembly, chromatin structure, chromatin modifying complexes, and RNA synthesis and regulation. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Comprehensive and accessible, Transcriptional Regulation: Methods and Protocols equally serves senior researchers and scientists experienced in transcriptional regulation as well as graduate students and scientists who wish to study transcriptional regulation for the first time.

Index Medicus

Index Medicus
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2004
  • Total pages :229
  • ISBN : OSU:32436011091889
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Summary :

Analysis of Gene Expression Patterns of Mammalian Cells at Different Metabolic States

Analysis of Gene Expression Patterns of Mammalian Cells at Different Metabolic States
  • Author : Rashmi Hemant Korke
  • Publisher :Unknown
  • Release Date :2002
  • Total pages :358
  • ISBN : MINN:31951P007997027
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Summary :

A Handbook of Transcription Factors

A Handbook of Transcription Factors
  • Author : Timothy R. Hughes
  • Publisher :Unknown
  • Release Date :2011-05-10
  • Total pages :306
  • ISBN : 904819069X
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Summary : Transcription factors are the molecules that the cell uses to interpret the genome: they possess sequence-specific DNA-binding activity, and either directly or indirectly influence the transcription of genes. In aggregate, transcription factors control gene expression and genome organization, and play a pivotal role in many aspects of physiology and evolution. This book provides a reference for major aspects of transcription factor function, encompassing a general catalogue of known transcription factor classes, origins and evolution of specific transcription factor types, methods for studying transcription factor binding sites in vitro, in vivo, and in silico, and mechanisms of interaction with chromatin and RNA polymerase.

Transcriptional Regulation in Eukaryotes

Transcriptional Regulation in Eukaryotes
  • Author : Michael Carey,Michael F. Carey,Stephen T. Smale
  • Publisher :Unknown
  • Release Date :2000
  • Total pages :640
  • ISBN : 0879696354
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Summary : In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene and the biochemistry of a new transcription factor. This important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states.

Gene Regulation in Eukaryotes

Gene Regulation in Eukaryotes
  • Author : Edgar Wingender
  • Publisher :Unknown
  • Release Date :1993
  • Total pages :430
  • ISBN : UOM:39015029969725
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Summary : A much-needed guide through the overwhelming amount of literature in the field. Comprehensive and detailed, this book combines background information with the most recentinsights. It introduces current concepts, emphasizing the transcriptional control of genetic information. Moreover, it links data on the structure of regulatory proteins with basic cellular processes. Both advanced students and experts will find answers to such intriguing questions as: - How are programs of specific gene repertoires activated and controlled? - Which genes drive and control morphogenesis? - Which genes govern tissue-specific tasks? - How do hormones control gene expression in coordinating the activities of different tissues? An abundant number of clearly presented glossary terms facilitates understanding of the biological background. Speacial feature: over 2200 (!) literature references.

Cumulated Index Medicus

Cumulated Index Medicus
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1989
  • Total pages :229
  • ISBN : UIUC:30112023522698
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Summary :

Microarrays and Transcription Networks

Microarrays and Transcription Networks
  • Author : M. Francis Shannon
  • Publisher :Unknown
  • Release Date :2006-09
  • Total pages :130
  • ISBN : STANFORD:36105114454635
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Summary : While every cell of an organism has an identical genomic content, extremely complex networks exist to tailor the genomic output to the needs of that cell. This program of gene expression is different for every cell type and stage of development. In addition, the cell can respond to its environment by modulating its gene expression program in a fairly dramatic manner. For many decades gene transcription has been investigated in systems from bacteria to mammalian cells and along the way many landmark findings have set new paradigms that often apply across wide evolutionary distances. Studying individual genes, however, especially in mammalian systems has been a painstaking business and although we know the transcription activators and other complexes that control specific genes in minute detail, generalizing these findings has often proven to be difficult. It has become clear that transcription factors do not operate alone but form complex networks in the cell. If one component of this complexity is disturbed then there are repercussions across the entire network, but it has been impossible to study these networks until very recently. The advent of microarray technology within the last decade has revolutionized how we study gene transcription. There are several types of array technology that essentially screen for relative mRNA levels for many thousands of genes at once. We do not focus here on the technology as this has become routine and is available to many researchers. Microarray technology has given us the ability to measure the entire gene expression program of a cell in a single experiment and compare it to other cells thus allowing a global view of cell behaviour at the level of gene transcription. Expression profiling, as this endeavour has become known, is now a relatively simple undertaking and hundreds, probably thousands of papers have been published demonstrating the power of this technology. Expression profiling has been applied to many diverse biological problems and is also being developed as a method for disease diagnosis especially in the cancer classification field. There are constant improvements or modified uses of the technology that are allowing more and more high throughput experiments to be carried out.

Molecular Biology of the Cell

Molecular Biology of the Cell
  • Author : Bruce Alberts
  • Publisher :Unknown
  • Release Date :2004
  • Total pages :229
  • ISBN : 0815332181
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Summary :

Gene Expression and Regulation in Mammalian Cells

Gene Expression and Regulation in Mammalian Cells
  • Author : Fumiaki Uchiumi
  • Publisher :Unknown
  • Release Date :2018-02-21
  • Total pages :334
  • ISBN : 9789535138556
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Summary : "Central dogma" was presented by Dr. Francis Crick 60 years ago. The information of nucleotide sequences on DNAs is transcribed into RNAs by RNA polymerases. We learned the mechanisms of how transcription determines function of proteins and behaviour of cells and even how it brings appearances of organisms. This book is intended for scientists and medical researchers especially who are interested in the relationships between transcription and human diseases. This volume consists of an introductory chapter and 14 chapters, divided into 4 parts. Each chapter is written by experts in the basic scientific field. A collection of articles presented by active and laboratory-based investigators provides recent advances and progresses in the field of transcriptional regulation in mammalian cells.

Transcription Factor Protocols

Transcription Factor Protocols
  • Author : Martin J. Tymms
  • Publisher :Unknown
  • Release Date :2000
  • Total pages :318
  • ISBN : 9781592596867
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Summary : In Transcription Factor Protocols, Martin Tymms has created a powerful compendium of the major techniques for the study of those DNA sequences and protein factors that regulate the transcription of protein encoding genes. With chapters contributed by leading investigators well versed in the methods, this eminently practical compendium includes not only well established protocols, but also novel techniques that are now being widely adopted. Among the important new methods treated are the use of triplex-forming oligonucleotides, the application of whole genome PCR to the isolation of gene promoters/enhancers, the analysis of in vivo methylation, and in vivo footprinting using UV light and ligation-mediated PCR. Step-by-step instructions, tips about pitfalls to avoid, and extensive procedural notes all help to ensure robust and reproducible results.Transcription Factor Protocols offers both experienced workers and new researchers a vital first-stop reference collection of the core techniques for all those exploring the role of transcription factors in gene regulation today.

Research Awards Index

Research Awards Index
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :1984
  • Total pages :229
  • ISBN : STANFORD:36105119624752
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Summary :

Issues in General Science and Scientific Theory and Method: 2011 Edition

Issues in General Science and Scientific Theory and Method: 2011 Edition
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2012-01-09
  • Total pages :3278
  • ISBN : 9781464963476
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Summary : Issues in General Science and Scientific Theory and Method: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about General Science and Scientific Theory and Method. The editors have built Issues in General Science and Scientific Theory and Method: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about General Science and Scientific Theory and Method in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in General Science and Scientific Theory and Method: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Functional Analysis of Mammalian Mediator

Functional Analysis of Mammalian Mediator
  • Author : Gregory Toivo Cantin
  • Publisher :Unknown
  • Release Date :2003
  • Total pages :180
  • ISBN : UCLA:L0087458840
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Summary :

Cap-Analysis Gene Expression (CAGE)

Cap-Analysis Gene Expression (CAGE)
  • Author : Piero Carninci
  • Publisher :Unknown
  • Release Date :2019-05-08
  • Total pages :266
  • ISBN : 9789814241359
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Summary : This book is a guide for users of new technologies, as it includes accurately proven protocols, allowing readers to prepare their samples for experiments. Additionally, it is a guide for the bioinformatics tools that are available for the analysis of the obtained tags, including the design of the software, the sources and the Web. Finally, the book provides examples of the application of these technologies to identify promoters, annotate genomes, identify new RNAs and reconstruct models of transcriptional control. Although examples mainly concern mammalians, the discussion expands to other groups of eukaryotes, where these approaches are complementing genome sequencing.

Dissertation Abstracts International

Dissertation Abstracts International
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2008
  • Total pages :229
  • ISBN : STANFORD:36105131549649
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Summary :

Transcription Factor Regulatory Networks

Transcription Factor Regulatory Networks
  • Author : Etsuko Miyamoto-Sato,Hiroyuki Ohashi,Hirotaka Sasaki,Jun-ichi Nishikawa,Hiroshi Yanagawa
  • Publisher :Unknown
  • Release Date :2014-06-14
  • Total pages :220
  • ISBN : 1493908049
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Summary : Transcription Factor Regulatory Methods details various techniques ranging from cutting-edge to general techniques use to study transcription factor regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Transcription Factor Regulatory Methods aids scientists in the further study into post-genomic or the personal genomic era.

Engineering Mammalian Cells by Exploring Physiological Landscapes

Engineering Mammalian Cells by Exploring Physiological Landscapes
  • Author : Gargi Seth
  • Publisher :Unknown
  • Release Date :2006
  • Total pages :298
  • ISBN : MINN:31951P00875355R
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Summary :

Wiley Encyclopedia of Chemical Biology, Volume 4

Wiley Encyclopedia of Chemical Biology, Volume 4
  • Author : Tadhg P. Begley
  • Publisher :Unknown
  • Release Date :2009-02-03
  • Total pages :828
  • ISBN : STANFORD:36105210630856
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Summary : The first major reference at the interface of chemistry, biology, and medicine Chemical biology is a rapidly developing field that uses the principles, tools, and language of chemistry to answer important questions in the life sciences. It has enabled researchers to gather critical information about the molecular biology of the cell and is the fundamental science of drug discovery, playing a key role in the development of novel agents for the prevention, diagnosis, and treatment of disease. Now students and researchers across the range of disciplines that use chemical biology techniques have a single resource that encapsulates what is known in the field. It is an excellent place to begin any chemical biology investigation. Major topics addressed in the encyclopedia include: Applications of chemical biology Biomolecules within the cell Chemical views of biology Chemistry of biological processes and systems Synthetic molecules as tools for chemical biology Technologies and techniques in chemical biology Some 300 articles range from pure basic research to areas that have immediate applications in fields such as drug discovery, sensor technology, and catalysis. Novices in the field can turn to articles that introduce them to the basics, whereas experienced researchers have access to articles exploring the cutting edge of the science. Each article ends with a list of references to facilitate further investigation. With contributions from leading researchers and pioneers in the field, the Wiley Encyclopedia of Chemical Biology builds on Wiley's unparalleled reputation for helping students and researchers understand the crucial role of chemistry and chemical techniques in the life sciences.

Analysis of a Transcriptional Repressor in Drosophila Melanogaster

Analysis of a Transcriptional Repressor in Drosophila Melanogaster
  • Author : Yifan Mao
  • Publisher :Unknown
  • Release Date :2000
  • Total pages :200
  • ISBN : MSU:31293020741926
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Summary :