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Microfluidic Cell Culture Systems

Microfluidic Cell Culture Systems
  • Author : Christopher Bettinger,Jeffrey T Borenstein,Sarah L Tao
  • Publisher :Unknown
  • Release Date :2012-12-31
  • Total pages :452
  • ISBN : 9781437734607
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Summary : The fields of microfluidics and BioMEMS are significantly impacting cell biology research and applications through the application of engineering solutions to human disease and health problems. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology. This new professional reference applies the techniques of microsystems to cell culture applications. The authors provide a thoroughly practical guide to the principles of microfluidic device design and operation and their application to cell culture techniques. The resulting book is crammed with strategies and techniques that can be immediately deployed in the lab. Equally, the insights into cell culture applications will provide those involved in traditional microfluidics and BioMEMS with an understanding of the specific demands and opportunities presented by biological applications. The goal is to guide new and interested researchers and technology developers to the important areas and state-of-the-practice strategies that will enhance the efficiency and value of their technologies, devices and biomedical products. Provides insights into the design and development of microfluidic systems with a specific focus on cell culture applications Focuses on strategies and techniques for the design and fabrication of microfluidic systems and devices for cell culture Provides balanced coverage of microsystems engineering and bioengineering

Microfluidic Cell Culture Systems

Microfluidic Cell Culture Systems
  • Author : Christopher Bettinger,Jeffrey T. Borenstein,Sarah L. Tao
  • Publisher :Unknown
  • Release Date :2013
  • Total pages :433
  • ISBN : 1437734596
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Summary : The fields of microfluidics and BioMEMS are signigicantly impacting cell biology research and applications through the application of engineering solutions to human disease and health problems. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology. One of the key benefits of microfluidics for bioengineering is the ability to control parameters of the cell microenvironment at relevant length and time scales. This new professional reference applies the techniques of microsystems to cell culture applications. The authors provide a thoroughly practical guide to the principles of microfluidic device design and operation, and their application to cell culture techniques. The resulting book is crammed with strategies and techniques that can be immediately deployed in the lab. Equally, the insights into cell culture applications will provide those involved in traditional microfluidics and bioMEMS with an understanding of the specific demands and opportunities presented by biological applications. The goal is to guide new and interested researchers and technology developers to the important areas and state-of-the-practice strategies that will enhance the efficiency and value of their technolgies, devices and biomedical products. Provides insights into the design and development of microfluidic systems with a specific focus on cell culture applications. Focuses on strategies and techniques for the design and fabrication of microfluidic systems and devices for cell culture. Provides a balanced coverage of microsystems engineering and bioengineering.

Exam Prep for: Microfluidic Cell Culture Systems

Exam Prep for: Microfluidic Cell Culture Systems
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2021
  • Total pages :229
  • ISBN : 1230987654XX
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Summary :

Cardiac Cell Culture Technologies

Cardiac Cell Culture Technologies
  • Author : Zbigniew Brzozka,Elzbieta Jastrzebska
  • Publisher :Unknown
  • Release Date :2017-11-21
  • Total pages :234
  • ISBN : 9783319706856
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Summary : This book provides an introduction to the biological background of heart functioning and analyzes the various materials and technologies used for the development of microfluidic systems dedicated to cell culture, with an emphasis on cardiac cells. The authors describe the characterization of microfluidic systems for cardiac cell culture and center their discussion of the use of stem cell stimulation based on four different types: electrical, biochemical, physical, and mechanical. This book is appropriate for researchers focused on on-chip technologies and heart studies, students in bioengineering and microengineering courses, and a variety of professionals, such as biotechnologists, biomedical engineers, and clinicians working in the cardiac diseases field.

Microfluidics for Single-Cell Analysis

Microfluidics for Single-Cell Analysis
  • Author : Jin-Ming Lin
  • Publisher :Unknown
  • Release Date :2019-08-28
  • Total pages :261
  • ISBN : 9789813297296
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Summary : This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Advances in Microfluidics

Advances in Microfluidics
  • Author : Xiao-Ying Yu
  • Publisher :Unknown
  • Release Date :2016-11-23
  • Total pages :422
  • ISBN : 9789535127857
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Summary : Increasing innovations and applications make microfluidics a versatile choice for researchers in many disciplines. This book consists of multiple review chapters that aim to cover recent advances and new applications of microfluidics in biology, electronics, energy, and materials sciences. It provides comprehensive views of various aspects of microfluidics ranging from fundamentals of fabrication, flow control, and droplet manipulation to the most recent exploration in emerging areas such as material synthesis, imaging and novel spectroscopy, and marriage with electronics. The chapters have many illustrations showcasing exciting results. This book should be useful for those who are eager to learn more about microfluidics as well as researchers who want to pick up new concepts and developments in this fast-growing field.

Organ-on-a-chip

Organ-on-a-chip
  • Author : Julia Hoeng,David Bovard,Manuel C. Peitsch
  • Publisher :Unknown
  • Release Date :2019-11-09
  • Total pages :546
  • ISBN : 9780128172032
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Summary : Organ-on-a-Chip: Engineered Microenvironments for Safety and Efficacy Testing contains chapters from world-leading researchers in the field of organ on a chip development and applications, with perspectives from life sciences, medicine, physiology and engineering. The book contains an overview of the field, with sections covering the major organ systems and currently available technologies, platforms and methods. As readers may also be interested in creating biochips, materials and engineering best practice, these topics are also described. Users will learn about the limitations of 2D in-vitro models and the available 3D in-vitro models (what benefits they offer and some examples). Finally, the MOC section shows how the organ on a chip technology can be adapted to improve the physiology of in-vitro models. Includes case studies of other organs on a chip that have been developed and successfully used Provides insights into functional microphysiological organ on a chip platforms for toxicity and efficacy testing, along with opportunities for translational medicine Presented fields (PK/PD, physiology, medicine, safety) are given a definition followed by the challenges and potential of organs on a chip

Animal Biotechnology

Animal Biotechnology
  • Author : Ashish S. Verma,Anchal Singh
  • Publisher :Unknown
  • Release Date :2020-07-03
  • Total pages :798
  • ISBN : 9780128117101
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Summary : Animal Biotechnology: Models in Discovery and Translation, Second Edition, provides a helpful guide to anyone seeking a thorough review of animal biotechnology and its application to human disease and welfare. This updated edition covers vital fundamentals, including animal cell cultures, genome sequencing analysis, epigenetics and animal models, gene expression, and ethics and safety concerns, along with in-depth examples of implications for human health and prospects for the future. New chapters cover animal biotechnology as applied to various disease types and research areas, including in vitro fertilization, human embryonic stem cell research, biosensors, enteric diseases, biopharming, organ transplantation, tuberculosis, neurodegenerative disorders, and more. Highlights the latest biomedical applications of genetically modified and cloned animals, with a focus on cancer and infectious diseases Offers first-hand accounts of the use of biotechnology tools, including molecular markers, stem cells, animal cultures, tissue engineering, ADME and CAM Assay Includes case studies that illustrate safety assessment issues, ethical considerations, and intellectual property rights associated with the translation of animal biotechnology studies

Microfluidic Cell Culture Systems

Microfluidic Cell Culture Systems
  • Author : Jeffrey T Borenstein,Vishal Tandon,Sarah L Tao,Joseph L. Charest
  • Publisher :Unknown
  • Release Date :2018-09-12
  • Total pages :396
  • ISBN : 9780128136720
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Summary : Techniques for microfabricating intricate microfluidic structures that mimic the microenvironment of tissues and organs, combined with the development of biomaterials with carefully engineered surface properties, have enabled new paradigms in and cell culture-based models for human diseases. The dimensions of surface features and fluidic channels made accessible by these techniques are well-suited to the size scale of biological cells. Microfluidic Cell Culture Systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organ-on-chip systems. This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. The book covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. The goal is to help professionals coming from an engineering background to adapt their expertise for use in cell culture and organ models applications, and likewise to help biologists to design and employ microfluidic technologies in their cell culture systems. This 2nd edition contains new material that strengthens the focus on in vitro models useful for drug discovery and development. One new chapter reviews liver organ models from an industry perspective, while others cover new technologies for scaling these models and for multi-organ systems. Other new chapters highlight the development of organ models and systems for specific applications in disease modeling and drug safety. Previous chapters have been revised to reflect the latest advances. Provides design and operation methodology for microfluidic and microfabricated materials and devices for organ-on-chip disease and safety models. This is a rapidly expanding field that will continue to grow along with advances in cell biology and microfluidics technologies. Comprehensively covers strategies and techniques ranging from academic first principles to industrial scale-up approaches. Readers will gain insight into cell-material interactions, microfluidic flow, and design principles. Offers three fundamental types of information: 1) design principles, 2) operation techniques, and 3) background information/perspectives. The book is carefully designed to strike a balance between these three areas, so it will be of use to a broad range of readers with different technical interests and educational levels.

Microfluidics in Cell BiologyPart A: Microfluidics for multicellular systems

Microfluidics in Cell BiologyPart A: Microfluidics for multicellular systems
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2018-07-20
  • Total pages :273
  • ISBN : 9780128142813
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Summary : Microfluidics in Cell Biology Part A: Volume 146, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections on Cell monolayers/spheroids, Collective migration in microtubes, Leukocyte adhesion dynamics on endothelial monolayers under flow, Constrained spheroid for perfusion culture, Cells in droplet arrays, Heart on chips, Kidney on chips, Liver on chips and hepatic immune responses, Gut on chips, 3D microvascular model-based lymphoma model, Blood brain barrier on chips, Multi-organ-on-a-chip for pharmacokinetic analysis, Cancer immunotherapy on chips, and more. Contains contributions from experts in the field from across the globe Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Osteochondral Tissue Engineering

Osteochondral Tissue Engineering
  • Author : J. Miguel Oliveira,Sandra Pina,Rui L. Reis,Julio San Roman
  • Publisher :Unknown
  • Release Date :2018-05-07
  • Total pages :511
  • ISBN : 9783319767352
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Summary : This book covers the most recent developments in the field of osteochondral tissue engineering (OCTE) and covers in detail the concepts and current challenges for bone and cartilage repair and regeneration. Specific topics include viscosupplementation, biologicals, tissue engineering approaches, in vitro and in vivo models, and technological advances with stem cells, bioreactors, and microfluidics. Osteochondral Tissue Engineering: Challenges, Current Strategies, and Technological Advances presents challenges and strategies in the field of osteochondral regeneration and serves as a core reference for biomedical engineering students and a wide range of established researchers and professionals working in orthopedics.

Cell Culture Technology

Cell Culture Technology
  • Author : Cornelia Kasper,Verena Charwat,Antonina Lavrentieva
  • Publisher :Unknown
  • Release Date :2018-10-10
  • Total pages :168
  • ISBN : 9783319748542
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Summary : This textbook provides an overview on current cell culture techniques, conditions, and applications specifically focusing on human cell culture. This book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University of Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover, complemented by contributions from international experts, and therefore delivers in a compact and clear way important theoretical, as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. The book is written for Master students and PhD candidates in biotechnology, tissue engineering and biomedicine working with mammalian, and specifically human cells. It will be of interest to doctoral colleges, Master- and PhD programs teaching courses in this area of research.

Applications of Microfluidic Systems in Biology and Medicine

Applications of Microfluidic Systems in Biology and Medicine
  • Author : Manabu Tokeshi
  • Publisher :Unknown
  • Release Date :2019-04-25
  • Total pages :382
  • ISBN : 9789811362293
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Summary : This book focuses on state-of-the-art microfluidic research in medical and biological applications. The top-level researchers in this research field explain carefully and clearly what can be done by using microfluidic devices. Beginners in the field —undergraduates, engineers, biologists, medical researchers—will easily learn to understand microfluidic-based medical and biological applications. Because a wide range of topics is summarized here, it also helps experts to learn more about fields outside their own specialties. The book covers many interesting subjects, including cell separation, protein crystallization, single-cell analysis, cell diagnosis, point-of-care testing, immunoassay, embyos/worms on a chip and organ-on-a-chip. Readers will be convinced that microfluidic devices have great potential for medical and biological applications.

Lab-on-a-Chip Fabrication and Application

Lab-on-a-Chip Fabrication and Application
  • Author : Margarita Stoytcheva,Roumen Zlatev
  • Publisher :Unknown
  • Release Date :2016-06-29
  • Total pages :208
  • ISBN : 9789535124573
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Summary : The necessity of on-site, fast, sensitive, and cheap complex laboratory analysis, associated with the advances in the microfabrication technologies and the microfluidics, made it possible for the creation of the innovative device lab-on-a-chip (LOC), by which we would be able to scale a single or multiple laboratory processes down to a chip format. The present book is dedicated to the LOC devices from two points of view: LOC fabrication and LOC application.

Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis

Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2018-11-22
  • Total pages :264
  • ISBN : 9780128142851
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Summary : Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Microfluidic Methods for Molecular Biology

Microfluidic Methods for Molecular Biology
  • Author : Chang Lu,Scott S. Verbridge
  • Publisher :Unknown
  • Release Date :2016-05-14
  • Total pages :376
  • ISBN : 9783319300191
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Summary : This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry. This book also: Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics

Cell Analysis on Microfluidics

Cell Analysis on Microfluidics
  • Author : Jin-Ming Lin
  • Publisher :Unknown
  • Release Date :2017-10-25
  • Total pages :429
  • ISBN : 9789811053948
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Summary : This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.

Integrated Microfluidic Platforms for Quantitative Analysis of Cell Culture and Uptakes

Integrated Microfluidic Platforms for Quantitative Analysis of Cell Culture and Uptakes
  • Author : Tak For Yu
  • Publisher :Unknown
  • Release Date :2009
  • Total pages :230
  • ISBN : UCLA:L0099901613
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Summary :

Drug Testing In Vitro

Drug Testing In Vitro
  • Author : Uwe Marx,Volker Sandig
  • Publisher :Unknown
  • Release Date :2007-02-27
  • Total pages :318
  • ISBN : 9783527609567
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Summary : Here, expert scientists from industry and academia share their knowledge on the assembly of functional human tissues in vitro and how to design drug screenings predictive of human exposure. In so doing, they combine the latest technological developments with strategic outlooks, such as novel cell and tissue systems for drug screening and testing, as well as emerging in vitro culture technologies. Equally importantly, the book does not shy away from regulatory acceptance and ethical issues.

Microfluidic Devices for Biomedical Applications

Microfluidic Devices for Biomedical Applications
  • Author : Xiujun James Li,Yu Zhou
  • Publisher :Unknown
  • Release Date :2013-10-31
  • Total pages :676
  • ISBN : 9780857097040
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Summary : Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic devices for biomedical applications discusses the fundamentals of microfluidics and explores in detail a wide range of medical applications. The first part of the book reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focussing on the materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles. Part three considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The final part of the book covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis. Microfluidic devices for biomedical applications is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries. Discusses the fundamentals of microfluidics or lab-on-a-chip (LOC) and explores in detail a wide range of medical applications Considers materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies Considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering

Microfluidics Based Microsystems

Microfluidics Based Microsystems
  • Author : S. Kakaç,B. Kosoy,D. Li,A. Pramuanjaroenkij
  • Publisher :Unknown
  • Release Date :2010-06-30
  • Total pages :618
  • ISBN : 9789048190294
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Summary : This volume contains an archival record of the NATO Advanced Study Institute on Microfluidics Based Microsystems – Fundamentals and App- cations held in Çe ?me-Izmir, Turkey, August 23–September 4, 2009. ASIs are intended to be high-level teaching activity in scientific and technical areas of current concern. In this volume, the reader may find interesting chapters and various microsystems fundamentals and applications. As the world becomes increasingly concerned with terrorism, early - spot detection of terrorist’s weapons, particularly bio-weapons agents such as bacteria and viruses are extremely important. NATO Public Diplomacy division, Science for Peace and Security section support research, Advanced Study Institutes and workshops related to security. Keeping this policy of NATO in mind, we made such a proposal on Microsystems for security. We are very happy that leading experts agreed to come and lecture in this important NATO ASI. We will see many examples that will show us Microfluidics usefulness for rapid diagnostics following a bioterrorism attack. For the applications in national security and anti-terrorism, microfluidic system technology must meet the challenges. To develop microsystems for security and to provide a comprehensive state-of-the-art assessment of the existing research and applications by treating the subject in considerable depth through lectures from eminent professionals in the field, through discussions and panel sessions are very beneficial for young scientists in the field.