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Microscope Image Processing

Microscope Image Processing
  • Author : Qiang Wu,Fatima Merchant,Kenneth Castleman
  • Publisher :Unknown
  • Release Date :2010-07-27
  • Total pages :576
  • ISBN : 0080558542
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Summary : Digital image processing, an integral part of microscopy, is increasingly important to the fields of medicine and scientific research. This book provides a unique one-stop reference on the theory, technique, and applications of this technology. Written by leading experts in the field, this book presents a unique practical perspective of state-of-the-art microscope image processing and the development of specialized algorithms. It contains in-depth analysis of methods coupled with the results of specific real-world experiments. Microscope Image Processing covers image digitization and display, object measurement and classification, autofocusing, and structured illumination. Key Features: Detailed descriptions of many leading-edge methods and algorithms In-depth analysis of the method and experimental results, taken from real-life examples Emphasis on computational and algorithmic aspects of microscope image processing Advanced material on geometric, morphological, and wavelet image processing, fluorescence, three-dimensional and time-lapse microscopy, microscope image enhancement, MultiSpectral imaging, and image data management This book is of interest to all scientists, engineers, clinicians, post-graduate fellows, and graduate students working in the fields of biology, medicine, chemistry, pharmacology, and other related fields. Anyone who uses microscopes in their work and needs to understand the methodologies and capabilities of the latest digital image processing techniques will find this book invaluable. Presents a unique practical perspective of state-of-the-art microcope image processing and the development of specialized algorithms Each chapter includes in-depth analysis of methods coupled with the results of specific real-world experiments Co-edited by Kenneth R. Castleman, world-renowned pioneer in digital image processing and author of two seminal textbooks on the subject

Computer Vision for Microscopy Image Analysis

Computer Vision for Microscopy Image Analysis
  • Author : Mei Chen
  • Publisher :Unknown
  • Release Date :2020-12-01
  • Total pages :228
  • ISBN : 9780128149737
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Summary : Are you a computer scientist working on image analysis? Are you a biologist seeking tools to process the microscopy data from image-based experiments? Computer Vision for Microscopy Image Analysis provides a comprehensive and in-depth discussion of modern computer vision techniques, in particular deep learning, for microscopy image analysis that will advance your efforts. Progress in imaging techniques has enabled the acquisition of large volumes of microscopy data and made it possible to conduct large-scale, image-based experiments for biomedical discovery. The main challenge and bottleneck in such experiments is the conversion of "big visual data" into interpretable information. Visual analysis of large-scale microscopy data is a daunting task. Computer vision has the potential to automate this task. One key advantage is that computers perform analysis more reproducibly and less subjectively than human annotators. Moreover, high-throughput microscopy calls for effective and efficient techniques as there are not enough human resources to advance science by manual annotation. This book articulates the strong need for biologists and computer vision experts to collaborate to overcome the limits of human visual perception, and devotes a chapter each to the major steps in analyzing microscopy images, such as detection and segmentation, classification, tracking, and event detection. Discover how computer vision can automate and enhance the human assessment of microscopy images for discovery Grasp the state-of-the-art approaches, especially deep neural networks Learn where to obtain open-source datasets and software to jumpstart his or her own investigation

Content-based Microscopic Image Analysis

Content-based Microscopic Image Analysis
  • Author : Chen Li
  • Publisher :Unknown
  • Release Date :2016-05-15
  • Total pages :196
  • ISBN : 9783832542535
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Summary : In this dissertation, novel Content-based Microscopic Image Analysis (CBMIA) methods, including Weakly Supervised Learning (WSL), are proposed to aid biological studies. In a CBMIA task, noisy image, image rotation, and object recognition problems need to be addressed. To this end, the first approach is a general supervised learning method, which consists of image segmentation, shape feature extraction, classification, and feature fusion, leading to a semi-automatic approach. In contrast, the second approach is a WSL method, which contains Sparse Coding (SC) feature extraction, classification, and feature fusion, leading to a full-automatic approach. In this WSL approach, the problems of noisy image and object recognition are jointly resolved by a region-based classifier, and the image rotation problem is figured out through SC features. To demonstrate the usefulness and potential of the proposed methods, experiments are implemented on di erent practical biological tasks, including environmental microorganism classification, stem cell analysis, and insect tracking.

New Methods to Improve Large-Scale Microscopy Image Analysis with Prior Knowledge and Uncertainty

New Methods to Improve Large-Scale Microscopy Image Analysis with Prior Knowledge and Uncertainty
  • Author : Stegmaier, Johannes
  • Publisher :Unknown
  • Release Date :2017-02-08
  • Total pages :266
  • ISBN : 9783731505907
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Summary :

Metallographic and Materialographic Specimen Preparation, Light Microscopy, Image Analysis, and Hardness Testing

Metallographic and Materialographic Specimen Preparation, Light Microscopy, Image Analysis, and Hardness Testing
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2021
  • Total pages :229
  • ISBN : 1230987654XX
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Fundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging
  • Author : Douglas B. Murphy,Michael W. Davidson
  • Publisher :Unknown
  • Release Date :2012-08-22
  • Total pages :552
  • ISBN : 9781118382936
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Summary : Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy

Microscopic Image Analysis for Life Science Applications

Microscopic Image Analysis for Life Science Applications
  • Author : Jens Rittscher,Raghu Machiraju,Stephen T. C. Wong
  • Publisher :Unknown
  • Release Date :2008
  • Total pages :489
  • ISBN : 9781596932371
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Summary : This unique resource gives you a detailed understanding of imaging platforms, fluorescence imaging, and fundamental image processing algorithms. Further, it guides you through application of advanced image analysis methods and techniques to specific biological problems. The book presents applications that span a wide range of scales, from the detection of signaling events in sub-cellular structures, to the automated analysis of tissue structures.

Optical and Digital Image Processing

Optical and Digital Image Processing
  • Author : Gabriel Cristobal,Peter Schelkens,Hugo Thienpont
  • Publisher :Unknown
  • Release Date :2013-02-12
  • Total pages :988
  • ISBN : 9783527635252
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Summary : In recent years, Moore's law has fostered the steady growth of the field of digital image processing, though the computational complexity remains a problem for most of the digital image processing applications. In parallel, the research domain of optical image processing has matured, potentially bypassing the problems digital approaches were suffering and bringing new applications. The advancement of technology calls for applications and knowledge at the intersection of both areas but there is a clear knowledge gap between the digital signal processing and the optical processing communities. This book covers the fundamental basis of the optical and image processing techniques by integrating contributions from both optical and digital research communities to solve current application bottlenecks, and give rise to new applications and solutions. Besides focusing on joint research, it also aims at disseminating the knowledge existing in both domains. Applications covered include image restoration, medical imaging, surveillance, holography, etc... "a very good book that deserves to be on the bookshelf of a serious student or scientist working in these areas." Source: Optics and Photonics News

Computer Processing of Electron Microscope Images

Computer Processing of Electron Microscope Images
  • Author : P. W. Hawkes
  • Publisher :Unknown
  • Release Date :2011-12-27
  • Total pages :296
  • ISBN : 3642813836
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Summary : Towards the end of the 1960s, a number of quite different circumstances combined to launch a period of intense activity in the digital processing of electron micro graphs. First, many years of work on correcting the resolution-limiting aberrations of electron microscope objectives had shown that these optical impediments to very high resolution could indeed be overcome, but only at the cost of immense exper imental difficulty; thanks largely to the theoretical work of K. -J. Hanszen and his colleagues and to the experimental work of F. Thon, the notions of transfer func tions were beginning to supplant or complement the concepts of geometrical optics in electron optical thinking; and finally, large fast computers, capable of manipu lating big image matrices in a reasonable time, were widely accessible. Thus the idea that recorded electron microscope images could be improved in some way or rendered more informative by subsequent computer processing gradually gained ground. At first, most effort was concentrated on three-dimensional reconstruction, particu larly of specimens with natural symmetry that could be exploited, and on linear operations on weakly scattering specimens (Chap. l). In 1973, however, R. W. Gerchberg and W. O. Saxton described an iterative algorithm that in principle yielded the phase and amplitude of the electron wave emerging from a strongly scattering speci men.

Bioimage Data Analysis Workflows

Bioimage Data Analysis Workflows
  • Author : Kōta Miura,Nataša Sladoje
  • Publisher :Unknown
  • Release Date :2020-01-01
  • Total pages :170
  • ISBN : 9783030223861
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Digital Image Processing

Digital Image Processing
  • Author : Castleman
  • Publisher :Unknown
  • Release Date :2007-09
  • Total pages :686
  • ISBN : 8131712869
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Near-field Microscopy And Near-field Optics

Near-field Microscopy And Near-field Optics
  • Author : Courjon Daniel
  • Publisher :Unknown
  • Release Date :2003-03-12
  • Total pages :340
  • ISBN : 9781911298960
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Summary : Near-field optics studies the behaviour of light fields in the vicinity of matter, where light is structured in propagating and evanescent fields. Near-field optical microscopy is the straightforward application of near-field optics.This textbook provides an overview for undergraduates and anyone who has an interest in peculiar optical phenomena, and serves as a technical manual for engineers and researchers. It consists of 12 chapters dealing with the history of near-field optics, non-radiating optics, optical noise, inverse problems, theory, instrumentation and applications; there is an appendix including the basic elements of Fourier optics and Maxwell equations.

The Image Processing Handbook

The Image Processing Handbook
  • Author : John C. Russ
  • Publisher :Unknown
  • Release Date :2016-04-19
  • Total pages :885
  • ISBN : 1439840636
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Summary : Whether obtained by microscopes, space probes, or the human eye, the same basic tools can be applied to acquire, process, and analyze the data contained in images. Ideal for self study, The Image Processing Handbook, Sixth Edition, first published in 1992, raises the bar once again as the gold-standard reference on this subject. Using extensive new illustrations and diagrams, it offers a logically organized exploration of the important relationship between 2D images and the 3D structures they reveal. Provides Hundreds of Visual Examples in FULL COLOR! The author focuses on helping readers visualize and compare processing and measurement operations and how they are typically combined in fields ranging from microscopy and astronomy to real-world scientific, industrial, and forensic applications. Presenting methods in the order in which they would be applied in a typical workflow—from acquisition to interpretation—this book compares a wide range of algorithms used to: Improve the appearance, printing, and transmission of an image Prepare images for measurement of the features and structures they reveal Isolate objects and structures, and measure their size, shape, color, and position Correct defects and deal with limitations in images Enhance visual content and interpretation of details This handbook avoids dense mathematics, instead using new practical examples that better convey essential principles of image processing. This approach is more useful to develop readers’ grasp of how and why to apply processing techniques and ultimately process the mathematical foundations behind them. Much more than just an arbitrary collection of algorithms, this is the rare book that goes beyond mere image improvement, presenting a wide range of powerful example images that illustrate techniques involved in color processing and enhancement. Applying his 50-year experience as a scientist, educator, and industrial consultant, John Russ offers the benefit of his image processing expertise for fields ranging from astronomy and biomedical research to food science and forensics. His valuable insights and guidance continue to make this handbook a must-have reference.

Microscopy and Analysis

Microscopy and Analysis
  • Author : Stefan G. Stanciu
  • Publisher :Unknown
  • Release Date :2016-09-21
  • Total pages :442
  • ISBN : 9789535125785
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Summary : Microscopes represent tools of the utmost importance for a wide range of disciplines. Without them, it would have been impossible to stand where we stand today in terms of understanding the structure and functions of organelles and cells, tissue composition and metabolism, or the causes behind various pathologies and their progression. Our knowledge on basic and advanced materials is also intimately intertwined to the realm of microscopy, and progress in key fields of micro- and nanotechnologies critically depends on high-resolution imaging systems. This volume includes a series of chapters that address highly significant scientific subjects from diverse areas of microscopy and analysis. Authoritative voices in their fields present in this volume their work or review recent trends, concepts, and applications, in a manner that is accessible to a broad readership audience from both within and outside their specialist area.

Fundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging
  • Author : Douglas B. Murphy
  • Publisher :Unknown
  • Release Date :2002-06-18
  • Total pages :384
  • ISBN : 9780471234296
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Summary : Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscope-skills such as how to align microscope optics and perform image processing. Fundamentals of Light Microscopy and Electronic Imaging explores the basics of microscope design and use. The comprehensive material discusses the optical principles involved in diffraction and image formation in the light microscope, the basic modes of light microscopy, the components of modern electronic imaging systems, and the image processing operations necessary to acquire and prepare an image. Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics as: * Illuminators, filters, and isolation of specific wavelengths * Phase contrast and differential interference contrast * Properties of polarized light and polarization microscopy * Fluorescence and confocal laser scanning microscopy * Digital CCD microscopy and image processing Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions. An essential resource for both, experienced and novice microscopists.

Image Analysis

Image Analysis
  • Author : Donat P. Hader
  • Publisher :Unknown
  • Release Date :2000-08-23
  • Total pages :480
  • ISBN : 9781482273908
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Summary : Automatic image analysis has become an important tool in many fields of biology, medicine, and other sciences. Since the first edition of Image Analysis: Methods and Applications, the development of both software and hardware technology has undergone quantum leaps. For example, specific mathematical filters have been developed for quality enhanceme

Digital Microscopy

Digital Microscopy
  • Author : Greenfield Sluder,David E. Wolf
  • Publisher :Unknown
  • Release Date :2013-08-07
  • Total pages :672
  • ISBN : 9780124078925
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Summary : The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. Expands coverage to include discussion of confocal microscopy not found in the previous edition Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy
  • Author : James Pawley
  • Publisher :Unknown
  • Release Date :2006-06-02
  • Total pages :988
  • ISBN : 038725921X
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Summary : Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.

Automated Microscopic Image Analysis

Automated Microscopic Image Analysis
  • Author : Heinz Simon,Klaus Voss,Knut Wenzelides
  • Publisher :Unknown
  • Release Date :1984
  • Total pages :123
  • ISBN : CHI:27977544
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The Essential Guide to Image Processing

The Essential Guide to Image Processing
  • Author : Alan C. Bovik
  • Publisher :Unknown
  • Release Date :2009-07-08
  • Total pages :672
  • ISBN : 0080922511
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Summary : A complete introduction to the basic and intermediate concepts of image processing from the leading people in the field Up-to-date content, including statistical modeling of natural, anistropic diffusion, image quality and the latest developments in JPEG 2000 This comprehensive and state-of-the art approach to image processing gives engineers and students a thorough introduction, and includes full coverage of key applications: image watermarking, fingerprint recognition, face recognition and iris recognition and medical imaging. "This book combines basic image processing techniques with some of the most advanced procedures. Introductory chapters dedicated to general principles are presented alongside detailed application-orientated ones. As a result it is suitably adapted for different classes of readers, ranging from Master to PhD students and beyond." – Prof. Jean-Philippe Thiran, EPFL, Lausanne, Switzerland "Al Bovik’s compendium proceeds systematically from fundamentals to today’s research frontiers. Professor Bovik, himself a highly respected leader in the field, has invited an all-star team of contributors. Students, researchers, and practitioners of image processing alike should benefit from the Essential Guide." – Prof. Bernd Girod, Stanford University, USA "This book is informative, easy to read with plenty of examples, and allows great flexibility in tailoring a course on image processing or analysis." – Prof. Pamela Cosman, University of California, San Diego, USA A complete and modern introduction to the basic and intermediate concepts of image processing – edited and written by the leading people in the field An essential reference for all types of engineers working on image processing applications Up-to-date content, including statistical modelling of natural, anisotropic diffusion, image quality and the latest developments in JPEG 2000

Biomedical Image Processing and Three-dimensional Microscopy

Biomedical Image Processing and Three-dimensional Microscopy
  • Author : Rajgopal Sundaraj Acharya,Carol J. Cogswell,Dmitry B. Goldgof
  • Publisher :Unknown
  • Release Date :1992
  • Total pages :835
  • ISBN : CORNELL:31924067448989
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Summary :