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Separation Science and Proteomics

Separation Science and Proteomics
  • Author : Alexandre Stoyanov
  • Publisher :Unknown
  • Release Date :2017-06-01
  • Total pages :250
  • ISBN : 0128032618
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Summary : Separation Science and Proteomics: Current Trends and New Approaches provides readers with the most advanced theories and methodologies on the most important separation methods contributing to current proteomics. Intended to be a practical reference that meets the needs of everyday practitioners, it also serves as a go-to guide for scientists focused on the theory of separation science, new methods development, and new equipment design. Future progress in proteomics is connected to the introduction of new and more powerful techniques and the perfection of techniques proposed over the past several decades, and an in-depth understanding of physical mechanisms for a particular separation method or methods combination is crucial for scientists who need to bridge the gap between traditional and contemporary methods. Authored by a chemist who has more than 20 years of experience in separation science and proteomics, this book offers readers insights and solutions to the separation mechanism of a full range of related techniques and complex problems, including the concentration distribution of analyzed protein during IEF separation in natural or IPG gradients or detailed mechanisms of electrophoresis in thermal gradients. Covers theoretical aspects, such as resolving power limitations as flow rate and electric current density reach technical limits Provides a critical review of newly proposed separation methods and approaches, including a range of equilibrium gradient methods Addresses the physical aspects important for optimization of the separation process, including acid–base equilibria, dissociation schemes, and chemistry of non-aqueous solutions Analyzes biophysical methods of protein structure analysis and discusses scaling to micro-separations and possible applications of nanotechnologies

Separation Methods In Proteomics

Separation Methods In Proteomics
  • Author : Gary B. Smejkal,Alexander Lazarev
  • Publisher :Unknown
  • Release Date :2005-12-12
  • Total pages :536
  • ISBN : 9781420028058
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Summary : Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research. Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the optimal separation strategies to solve particular biological problems. Recent progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated “subproteomes" for individual analysis. It also highlights several hybrid approaches that take into account protein interactions. Including applications of the separation methods currently employed in proteomic analyses for both clinical and basic research, Separation Methods in Proteomics contains practical information that can enhance the current and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.

Polymer Monoliths Photopatterned Within Microfluidic Devices for Proteomics and Separation Science

Polymer Monoliths Photopatterned Within Microfluidic Devices for Proteomics and Separation Science
  • Author : Mei He
  • Publisher :Unknown
  • Release Date :2008
  • Total pages :322
  • ISBN : OCLC:826299398
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Summary :

Introducing Proteomics

Introducing Proteomics
  • Author : Josip Lovric
  • Publisher :Unknown
  • Release Date :2011-06-17
  • Total pages :296
  • ISBN : 9781119957195
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Summary : Introducing Proteomics gives a concise and coherent overview of every aspect of current proteomics technology, which is a rapidly developing field that is having a major impact within the life and medical sciences. This student-friendly book, based on a successful course developed by the author, provides its readers with sufficient theoretical background to be able to plan, prepare, and analyze a proteomics study. The text covers the following: Separation Technologies Analysis of Peptides/Proteins by Mass Spectrometry Strategies in Proteomics This contemporary text also includes numerous examples and explanations for why particular strategies are better than others for certain applications. In addition, Introducing Proteomics includes extensive references and a list of relevant proteomics information sources; essential for any student. This no-nonsense approach to the subject tells students exactly what they need to know, leaving out unnecessary information. The student companion site enhances learning and provides answers to the end of chapter problems. "I think this book will be a popular and valuable resource for students and newcomers to the field who would like to have an overview and initial understanding of what proteomics is about. The contents are well organized and address the major issues." —Professor Walter Kolch, Director, Systems Biology Ireland & Conway Institute, University College Dublin Companion Website www.wiley.com/go/lovric

Handbook of Isoelectric Focusing and Proteomics

Handbook of Isoelectric Focusing and Proteomics
  • Author : David Garfin,Satinder Ahuja
  • Publisher :Unknown
  • Release Date :2005-09-29
  • Total pages :380
  • ISBN : 9780080533629
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Summary : Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. * Is the only current book available on the subject * Includes author insights and practical tips * Is an ideal single source for students and researchers working in proteomics

Handbook of Isoelectric Focusing and Proteomics

Handbook of Isoelectric Focusing and Proteomics
  • Author : David Garfin,Satinder Ahuja
  • Publisher :Unknown
  • Release Date :2005-05-23
  • Total pages :334
  • ISBN : 0120887525
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Summary : Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. * Is the only current book available on the subject * Includes author insights and practical tips * Is an ideal single source for students and researchers working in proteomics

Gel-free Proteomics

Gel-free Proteomics
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2009
  • Total pages :137
  • ISBN : OCLC:634099775
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Summary :

Design and Synthesis of Advanced Materials for Separation Science

Design and Synthesis of Advanced Materials for Separation Science
  • Author : Michael Douglas Slater
  • Publisher :Unknown
  • Release Date :2009
  • Total pages :380
  • ISBN : UCAL:C3511321
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Summary :

Monolithic Silicas in Separation Science

Monolithic Silicas in Separation Science
  • Author : Klaus K. Unger,Nobuo Tanaka,Egidijus Machtejevas
  • Publisher :Unknown
  • Release Date :2010-11-29
  • Total pages :362
  • ISBN : 9783527633265
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Summary : Edited by the experts and pioneers in the field, this is the first monograph to cover the topic, containing the must-have information hitherto only scattered among journals. Clearly divided into sections on preparation, characterization and modeling, and applications, this is essential reading for chemists, chromatographers, analytical chemists, biochemists and biologists.

Introduction to Proteomics

Introduction to Proteomics
  • Author : Daniel Liebler
  • Publisher :Unknown
  • Release Date :2001-12-04
  • Total pages :198
  • ISBN : 0896039919
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Summary : Daniel C. Liebler masterfully introduces the science of proteomics by spelling out the basics of how one analyzes proteins and proteomes, and just how these approaches are then employed to investigate their roles in living systems. He explains the key concepts of proteomics, how the analytical instrumentation works, what data mining and other software tools do, and how these tools can be integrated to study proteomes. Also discussed are how protein and peptide separation techniques are applied in proteomics, how mass spectrometry is used to identify proteins, and how data analysis software enables protein identification and the mapping of modifications. In addition, there are proteomic approaches for analyzing differential protein expression, characterizing proteomic diversity, and dissecting protein-protein interactions and networks.

Proteomics in Food Science

Proteomics in Food Science
  • Author : Michelle Lisa Colgrave
  • Publisher :Unknown
  • Release Date :2017-04-03
  • Total pages :538
  • ISBN : 9780128040577
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Summary : Proteomics in Food Science: From Farm to Fork is a solid reference providing concepts and practical applications of proteomics for those in various disciplines of food science. The book covers a range of methods for elucidating the identity or composition of specific proteins in foods or cells related to food science, from spoilage organisms, to edible components. A variety of analytical platforms are described, ranging from the usage of simple electrophoresis, to more sophisticated mass spectrometry and bio-informatic platforms. The book is designed for food scientists, technologists, food industry workers, microbiologists, and public health workers, and can also be a valuable reference book for students. Includes a variety of analytical platforms, ranging from simple electrophoresis to more sophisticated mass spectrometry and bio-informatic platforms Presents analytical techniques for each food domain, including beverages, meats, dairy and eggs, fruit, fish/seafood, cereals, nuts, and grains that range from sample collection, proportion, and storage analysis Provides applications of proteomics in hot topics area of food safety, including food spoilage, pathogenic organisms, and allergens Covers major pathogens of concern e.g., Salmonella and applications to animal husbandry

Handbook of Pharmaceutical Biotechnology

Handbook of Pharmaceutical Biotechnology
  • Author : Shayne C. Gad
  • Publisher :Unknown
  • Release Date :2007-06-11
  • Total pages :1659
  • ISBN : UOM:39015064951935
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Summary : Describes the use of biotechnology to develop pharmaceuticals. This book gives the professional a basic tool to facilitate the development of biotech medicines by bringing together a general overview of biotechnology used in the drug development process, along with a compendium of regulations and validation methods.

Issues in Chemical, Biological, and Medical Engineering: 2013 Edition

Issues in Chemical, Biological, and Medical Engineering: 2013 Edition
  • Author : Anonim
  • Publisher :Unknown
  • Release Date :2013-05-01
  • Total pages :660
  • ISBN : 9781490108926
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Summary : Issues in Chemical, Biological, and Medical Engineering: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Biosystems Engineering. The editors have built Issues in Chemical, Biological, and Medical Engineering: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Biosystems Engineering in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Chemical, Biological, and Medical Engineering: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Proteome Characterization and Proteomics

Proteome Characterization and Proteomics
  • Author : Timothy D. Veenstra,Richard D. Smith
  • Publisher :Unknown
  • Release Date :2003-10-01
  • Total pages :413
  • ISBN : 0080569153
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Summary : The content of this volume is designed to reach a wide audience, including those involved with relevant technologies such as electrophoresis and mass spectrometry, to those interested in how proteomics can benefit research. A wide range of techniques are discussed, each specifically designed to address different needs in proteomic analysis. The concluding chapter discusses the important issue related to handling large amounts of data accumulated in proteomic studies. Discusses proteomics in the postgenomic age Includes various strategies for quantitative proteomics Covers the role of MS in structural functional proteomics and proteomics in drug discovery and bioinformatics

Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis

Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis
  • Author : Fangjun Wang
  • Publisher :Unknown
  • Release Date :2013-12-02
  • Total pages :94
  • ISBN : 9783642420085
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Summary : In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer’s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.

Characterization and Improvement of Two-dimensional Gel Electrophoresis

Characterization and Improvement of Two-dimensional Gel Electrophoresis
  • Author : Christina M. Bondy
  • Publisher :Unknown
  • Release Date :2005
  • Total pages :261
  • ISBN : WISC:89090406174
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Summary :

Proteomic and Metabolomic Approaches to Biomarker Discovery

Proteomic and Metabolomic Approaches to Biomarker Discovery
  • Author : Haleem J. Issaq,Timothy D. Veenstra
  • Publisher :Unknown
  • Release Date :2019-10-24
  • Total pages :504
  • ISBN : 9780128197882
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Summary : Proteomic and Metabolomic Approaches to Biomarker Discovery, Second Edition covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods and presents a standard operating procedure for sample selection, preparation and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process. This second edition has been fully updated and revised to address recent advances in MS and NMR instrumentation, high-field NMR, proteomics and metabolomics for biomarker validation, clinical assays of biomarkers and clinical MS and NMR, identifying microRNAs and autoantibodies as biomarkers, MRM-MS assay development, top-down MS, glycosylation-based serum biomarkers, cell surface proteins in biomarker discovery, lipodomics for cancer biomarker discovery, and strategies to design studies to identify predictive biomarkers in cancer research. Addresses the full range of proteomic and metabolomic methods and technologies used for biomarker discovery and validation Covers all steps involved in biomarker discovery, from study design to study execution Serves as a vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals and graduate students

The Protein Protocols Handbook

The Protein Protocols Handbook
  • Author : John M. Walker
  • Publisher :Unknown
  • Release Date :2009-10-07
  • Total pages :1991
  • ISBN : STANFORD:36105215315776
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Summary : The third edition of The Protein Protocols Handbook introduces 57 critically important new chapters, and significantly updates the previous edition's tried-and-trusted methods. The book offers over 200 key, readily reproducible protocols that ensure results.

Separation Methods In Proteomics

Separation Methods In Proteomics
  • Author : Gary B. Smejkal,Alexander Lazarev
  • Publisher :Unknown
  • Release Date :2005-12-12
  • Total pages :536
  • ISBN : 0824726995
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Summary : Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research. Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the optimal separation strategies to solve particular biological problems. Recent progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated “subproteomes" for individual analysis. It also highlights several hybrid approaches that take into account protein interactions. Including applications of the separation methods currently employed in proteomic analyses for both clinical and basic research, Separation Methods in Proteomics contains practical information that can enhance the current and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.

Platelet Proteomics

Platelet Proteomics
  • Author : Ángel García-Alonso,Yotis Senis
  • Publisher :Unknown
  • Release Date :2011-05-31
  • Total pages :424
  • ISBN : 9780470463376
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Summary : The purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics. This chapter shows potential applications for platelet proteomics not yet carried out. It includes examples of post-translational modifications (PTMs) analysis in platelets. The second part of the book focuses on the main research done so far on platelet proteomics. This includes general proteome mapping by non-gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel-based separation methods (2-DE), sub-proteome analyses (secretome/releasate, membrane proteins, organelles). Finally, the last section links the platelet transcriptome and application to disease. This section is highly relevant and includes chapters on proteomics, transcriptomics, functional genomics, systems biology, and their applications to platelet-related diseases.

Understanding Stress Response in Desulfovibrio Vulgaris Hildenborough

Understanding Stress Response in Desulfovibrio Vulgaris Hildenborough
  • Author : Alyssa Marie Redding
  • Publisher :Unknown
  • Release Date :2007
  • Total pages :582
  • ISBN : UCAL:C3510691
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Summary :